Cytochrome P450 3A4

NameCytochrome P450 3A4
Synonyms
  • 1,8-cineole 2-exo-monooxygenase
  • 1.14.13.-
  • Albendazole monooxygenase
  • Albendazole sulfoxidase
  • Cholesterol 25-hydroxylase
  • CYP3A3
  • CYPIIIA3
  • CYPIIIA4
  • Cytochrome P450 3A3
  • Cytochrome P450 HLp
  • Cytochrome P450 NF-25
  • Cytochrome P450-PCN1
  • Nifedipine oxidase
  • Quinine 3-monooxygenase
  • Taurochenodeoxycholate 6-alpha-hydroxylase
Gene NameCYP3A4
OrganismHuman
Amino acid sequence
>lcl|BSEQ0021936|Cytochrome P450 3A4
MALIPDLAMETWLLLAVSLVLLYLYGTHSHGLFKKLGIPGPTPLPFLGNILSYHKGFCMF
DMECHKKYGKVWGFYDGQQPVLAITDPDMIKTVLVKECYSVFTNRRPFGPVGFMKSAISI
AEDEEWKRLRSLLSPTFTSGKLKEMVPIIAQYGDVLVRNLRREAETGKPVTLKDVFGAYS
MDVITSTSFGVNIDSLNNPQDPFVENTKKLLRFDFLDPFFLSITVFPFLIPILEVLNICV
FPREVTNFLRKSVKRMKESRLEDTQKHRVDFLQLMIDSQNSKETESHKALSDLELVAQSI
IFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPNKAPPTYDTVLQMEYLDMVV
NETLRLFPIAMRLERVCKKDVEINGMFIPKGVVVMIPSYALHRDPKYWTEPEKFLPERFS
KKNKDNIDPYIYTPFGSGPRNCIGMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLSLG
GLLQPEKPVVLKVESRDGTVSGA
Number of residues503
Molecular Weight57342.67
Theoretical pI8.25
GO Classification
Functions
  • heme binding
  • quinine 3-monooxygenase activity
  • monooxygenase activity
  • taurochenodeoxycholate 6alpha-hydroxylase activity
  • steroid binding
  • aromatase activity
  • oxygen binding
  • caffeine oxidase activity
  • enzyme binding
  • steroid hydroxylase activity
  • vitamin D 24-hydroxylase activity
  • testosterone 6-beta-hydroxylase activity
  • oxidoreductase activity
  • vitamin D3 25-hydroxylase activity
  • iron ion binding
  • albendazole monooxygenase activity
Processes
  • calcitriol biosynthetic process from calciol
  • vitamin D metabolic process
  • exogenous drug catabolic process
  • steroid catabolic process
  • drug catabolic process
  • heterocycle metabolic process
  • monoterpenoid metabolic process
  • small molecule metabolic process
  • oxidative demethylation
  • oxidation-reduction process
  • steroid metabolic process
  • lipid metabolic process
  • alkaloid catabolic process
  • xenobiotic metabolic process
  • drug metabolic process
  • androgen metabolic process
Components
  • cytoplasm
  • endoplasmic reticulum membrane
  • integral component of membrane
  • intracellular membrane-bounded organelle
General FunctionVitamin d3 25-hydroxylase activity
Specific FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed:11159812). Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed:21576599).
Pfam Domain Function
Transmembrane Regions2-22
GenBank Protein IDNot Available
UniProtKB IDP08684
UniProtKB Entry NameCP3A4_HUMAN
Cellular LocationEndoplasmic reticulum membrane
Gene sequence
>lcl|BSEQ0021937|Cytochrome P450 3A4 (CYP3A4)
ATGGCTCTCATCCCAGACTTGGCCATGGAAACCTGGCTTCTCCTGGCTGTCAGCCTGGTG
CTCCTCTATCTATATGGAACCCATTCACATGGACTTTTTAAGAAGCTTGGAATTCCAGGG
CCCACACCTCTGCCTTTTTTGGGAAATATTTTGTCCTACCATAAGGGCTTTTGTATGTTT
GACATGGAATGTCATAAAAAGTATGGAAAAGTGTGGGGCTTTTATGATGGTCAACAGCCT
GTGCTGGCTATCACAGATCCTGACATGATCAAAACAGTGCTAGTGAAAGAATGTTATTCT
GTCTTCACAAACCGGAGGCCTTTTGGTCCAGTGGGATTTATGAAAAGTGCCATCTCTATA
GCTGAGGATGAAGAATGGAAGAGATTACGATCATTGCTGTCTCCAACCTTCACCAGTGGA
AAACTCAAGGAGATGGTCCCTATCATTGCCCAGTATGGAGATGTGTTGGTGAGAAATCTG
AGGCGGGAAGCAGAGACAGGCAAGCCTGTCACCTTGAAAGACGTCTTTGGGGCCTACAGC
ATGGATGTGATCACTAGCACATCATTTGGAGTGAACATCGACTCTCTCAACAATCCACAA
GACCCCTTTGTGGAAAACACCAAGAAGCTTTTAAGATTTGATTTTTTGGATCCATTCTTT
CTCTCAATAATCTTTCCATTCCTCATCCCAATTCTTGAAGTATTAAATATCTGTGTGTTT
CCAAGAGAAGTTACAAATTTTTTAAGAAAATCTGTAAAAAGGATGAAAGAAAGTCGCCTC
GAAGATACACAAAAGCACCGAGTGGATTTCCTTCAGCTGATGATTGACTCTCAGAATTCA
AAAGAAACTGAGTCCCACAAAGCTCTGTCCGATCTGGAGCTCGTGGCCCAATCAATTATC
TTTATTTTTGCTGGCTATGAAACCACGAGCAGTGTTCTCTCCTTCATTATGTATGAACTG
GCCACTCACCCTGATGTCCAGCAGAAACTGCAGGAGGAAATTGATGCAGTTTTACCCAAT
AAGGCACCACCCACCTATGATACTGTGCTACAGATGGAGTATCTTGACATGGTGGTGAAT
GAAACGCTCAGATTATTCCCAATTGCTATGAGACTTGAGAGGGTCTGCAAAAAAGATGTT
GAGATCAATGGGATGTTCATTCCCAAAGGGGTGGTGGTGATGATTCCAAGCTATGCTCTT
CACCGTGACCCAAAGTACTGGACAGAGCCTGAGAAGTTCCTCCCTGAAAGATTCAGCAAG
AAGAACAAGGACAACATAGATCCTTACATATACACACCCTTTGGAAGTGGACCCAGAAAC
TGCATTGGCATGAGGTTTGCTCTCATGAACATGAAACTTGCTCTAATCAGAGTCCTTCAG
AACTTCTCCTTCAAACCTTGTAAAGAAACACAGATCCCCCTGAAATTAAGCTTAGGAGGA
CTTCTTCAACCAGAAAAACCCGTTGTTCTAAAGGTTGAGTCAAGGGATGGCACCGTAAGT
GGAGCCTGA
GenBank Gene IDM18907
GeneCard IDNot Available
GenAtlas IDCYP3A4
HGNC IDHGNC:2637
Chromosome Location7
Locus7q21.1
References
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