Aspartate aminotransferase, cytoplasmic

NameAspartate aminotransferase, cytoplasmic
Synonyms
  • 2.6.1.1
  • cAspAT
  • cCAT
  • Cysteine aminotransferase, cytoplasmic
  • Cysteine transaminase, cytoplasmic
  • Glutamate oxaloacetate transaminase 1
  • Transaminase A
Gene NameGOT1
OrganismHuman
Amino acid sequence
>lcl|BSEQ0037039|Aspartate aminotransferase, cytoplasmic
MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQK
IANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFL
ARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLEN
APEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWA
IRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPP
AQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGM
FSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ
Number of residues413
Molecular Weight46247.14
Theoretical pI7.01
GO Classification
Functions
  • L-aspartate
  • L-phenylalanine
  • identical protein binding
  • carboxylic acid binding
  • L-cysteine
  • pyridoxal phosphate binding
  • phosphatidylserine decarboxylase activity
Processes
  • oxaloacetate metabolic process
  • cellular amino acid biosynthetic process
  • glutamate metabolic process
  • cellular nitrogen compound metabolic process
  • small molecule metabolic process
  • aspartate biosynthetic process
  • aspartate metabolic process
  • carbohydrate metabolic process
  • glutamate catabolic process to 2-oxoglutarate
  • glucose metabolic process
  • glutamate catabolic process to aspartate
  • response to glucocorticoid
  • polyamine metabolic process
  • fatty acid homeostasis
  • cellular response to insulin stimulus
  • glycerol biosynthetic process
  • sulfur amino acid metabolic process
  • L-methionine biosynthetic process from methylthioadenosine
  • aspartate catabolic process
  • Notch signaling pathway
  • gluconeogenesis
  • 2-oxoglutarate metabolic process
Components
  • cytosol
  • extracellular exosome
  • axon terminus
  • cytoplasm
  • nucleus
  • lysosome
General FunctionPyridoxal phosphate binding
Specific FunctionBiosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID179067
UniProtKB IDP17174
UniProtKB Entry NameAATC_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0010371|Aspartate aminotransferase, cytoplasmic (GOT1)
ATGGCACCTCCGTCAGTCTTTGCCGAGGTTCCGCAGGCCCAGCCTGTCCTGGTCTTCAAG
CTCACTGCCGACTTCAGGGAGGATCCGGACCCCCGCAAGGTCAACCTGGGAGTGGGAGCA
TATCGCACGGATGACTGCCATCCCTGGGTTTTGCCAGTAGTGAAGAAAGTGGAGCAGAAG
ATTGCTAATGACAATAGCCTAAATCACGAGTATCTGCCAATCCTGGGCCTGGCTGAGTTC
CGGAGCTGTGCTTCTCGTCTTGCCCTTGGGGATGACAGCCCAGCACTCAAGGAGAAGCGG
GTAGGAGGTGTGCAATCTTTGGGGGGAACAGGTGCACTTCGAATTGGAGCTGATTTCTTA
GCGCGTTGGTACAATGGAACAAACAACAAGAACACACCTGTCTATGTGTCCTCACCAACC
TGGGAGAATCACAATGCTGTGTTTTCCGCTGCTGGTTTTAAAGACATTCGGTCCTATCGC
TACTGGGATGCAGAGAAGAGAGGATTGGACCTCCAGGGCTTCCTGAATGATCTGGAGAAT
GCTCCTGAGTTCTCCATTGTTGTCCTCCACGCCTGTGCACACAACCCAACTGGGATTGAC
CCAACTCCGGAGCAGTGGAAGCAGATTGCTTCTGTCATGAAGCACCGGTTTCTGTTCCCC
TTCTTTGACTCAGCCTATCAGGGCTTCGCATCTGGAAACCTGGAGAGAGATGCCTGGGCC
ATTCGCTATTTTGTGTCTGAAGGCTTCGAGTTCTTCTGTGCCCAGTCCTTCTCCAAGAAC
TTCGGGCTCTACAATGAGAGAGTCGGGAATCTGACTGTGGTTGGAAAAGAACCTGAGAGC
ATCCTGCAAGTCCTTTCCCAGATGGAGAAGATCGTGCGGATTACTTGGTCCAATCCCCCC
GCCCAGGGAGCACGAATTGTGGCCAGCACCCTCTCTAACCCTGAGCTCTTTGAGGAATGG
ACAGGTAATGTGAAGACAATGGCTGACCGGATTCTGACCATGAGATCTGAACTCAGGGCA
CGACTAGAAGCCCTCAAAACCCCTGGGACCTGGAACCACATCACTGATCAAATTGGCATG
TTCAGCTTCACTGGGTTGAACCCCAAGCAGGTTGAGTATCTGGTCAATGAAAAGCACATC
TACCTGCTGCCAAGTGGTCGAATCAACGTGAGTGGCTTAACCACCAAAAATCTAGATTAC
GTGGCCACCTCCATCCATGAAGCAGTCACCAAAATCCAGTGA
GenBank Gene IDM37400
GeneCard IDNot Available
GenAtlas IDGOT1
HGNC IDHGNC:4432
Chromosome Location10
Locus10q24.1-q25.1
References
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