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NameSerine/threonine-protein kinase Nek2
Synonyms
  • 2.7.11.1
  • HSPK 21
  • NEK2A
  • Never in mitosis A-related kinase 2
  • NimA-like protein kinase 1
  • NimA-related protein kinase 2
  • NLK1
Gene NameNEK2
OrganismHuman
Amino acid sequence
>lcl|BSEQ0007282|Serine/threonine-protein kinase Nek2
MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLR
ELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQYLDEEFVLRVMTQL
TLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTP
YYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRIPYRY
SDELNEIITRMLNLKDYHRPSVEEILENPLIADLVADEQRRNLERRGRQLGEPEKSQDSS
PVLSELKLKEIQLQERERALKAREERLEQKEQELCVRERLAEDKLARAENLLKNYSLLKE
RKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAA
QLRAQALSDIEKNYQLKSRQILGMR
Number of residues445
Molecular Weight51762.93
Theoretical pI9.25
GO Classification
Functions
  • ATP binding
  • protein phosphatase binding
  • protein kinase activity
  • protein serine/threonine kinase activity
  • metal ion binding
Processes
  • positive regulation of telomere maintenance via telomerase
  • mitotic nuclear division
  • mitotic spindle assembly
  • spindle assembly
  • protein phosphorylation
  • regulation of mitotic centrosome separation
  • G2/M transition of mitotic cell cycle
  • mitotic cell cycle
  • organelle organization
  • blastocyst development
  • protein autophosphorylation
  • meiotic cell cycle
  • regulation of mitotic nuclear division
  • negative regulation of DNA binding
  • mitotic sister chromatid segregation
  • chromosome segregation
  • regulation of attachment of spindle microtubules to kinetochore
  • anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
  • centrosome separation
  • negative regulation of centriole-centriole cohesion
  • positive regulation of telomerase activity
  • cell division
  • positive regulation of telomere capping
Components
  • cytosol
  • nucleolus
  • spindle pole
  • midbody
  • condensed nuclear chromosome
  • condensed chromosome kinetochore
  • kinetochore
  • protein complex
  • microtubule
  • centrosome
General FunctionProtein serine/threonine kinase activity
Specific FunctionProtein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID479171
UniProtKB IDP51955
UniProtKB Entry NameNEK2_HUMAN
Cellular LocationNucleus
Gene sequence
>lcl|BSEQ0021372|Serine/threonine-protein kinase Nek2 (NEK2)
ATGCCTTCCCGGGCTGAGGACTATGAAGTGTTGTACACCATTGGCACAGGCTCCTACGGC
CGCTGCCAGAAGATCCGGAGGAAGAGTGATGGCAAGATATTAGTTTGGAAAGAACTTGAC
TATGGCTCCATGACAGAAGCTGAGAAACAGATGCTTGTTTCTGAAGTGAATTTGCTTCGT
GAACTGAAACATCCAAACATCGTTCGTTACTATGATCGGATTATTGACCGGACCAATACA
ACACTGTACATTGTAATGGAATATTGTGAAGGAGGGGATCTGGCTAGTGTAATTACAAAG
GGAACCAAGGAAAGGCAATACTTAGATGAAGAGTTTGTTCTTCGAGTGATGACTCAGTTG
ACTCTGGCCCTGAAGGAATGCCACAGACGAAGTGATGGTGGTCATACCGTATTGCATCGG
GATCTGAAACCAGCCAATGTTTTCCTGGATGGCAAGCAAAACGTCAAGCTTGGAGACTTT
GGGCTAGCTAGAATATTAAACCATGACACGAGTTTTGCAAAAACATTTGTTGGCACACCT
TATTACATGTCTCCTGAACAAATGAATCGCATGTCCTACAATGAGAAATCAGATATCTGG
TCATTGGGCTGCTTGCTGTATGAGTTATGTGCATTAATGCCTCCATTTACAGCTTTTAGC
CAGAAAGAACTCGCTGGGAAAATCAGAGAAGGCAAATTCAGGCGAATTCCATACCGTTAC
TCTGATGAATTGAATGAAATTATTACGAGGATGTTAAACTTAAAGGATTACCATCGACCT
TCTGTTGAAGAAATTCTTGAGAACCCTTTAATAGCAGATTTGGTTGCAGACGAGCAAAGA
AGAAATCTTGAGAGAAGAGGGCGACAATTAGGAGAGCCAGAAAAATCGCAGGATTCCAGC
CCTGTATTGAGTGAGCTGAAACTGAAGGAAATTCAGTTACAGGAGCGAGAGCGAGCTCTC
AAAGCAAGAGAAGAAAGATTGGAGCAGAAAGAACAGGAGCTTTGTGTTCGTGAGAGACTA
GCAGAGGACAAACTGGCTAGAGCAGAAAATCTGTTGAAGAACTACAGCTTGCTAAAGGAA
CGGAAGTTCCTGTCTCTGGCAAGTAATCCAGAGTCTCACTTTGTTGCCCAGGCTGGAATG
CAGTGGTGTGATCACAGCTCAATGTAG
GenBank Gene IDZ29066
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:7745
Chromosome Location1
Locus1q32.2-q41
References
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  2. Hames RS, Fry AM: Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis. Biochem J. 2002 Jan 1;361(Pt 1):77-85. 11742531
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  8. Faragher AJ, Fry AM: Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles. Mol Biol Cell. 2003 Jul;14(7):2876-89. Epub 2003 Apr 17. 12857871
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