Ubiquitin-conjugating enzyme E2 N

NameUbiquitin-conjugating enzyme E2 N
Synonyms
  • 2.3.2.23
  • Bendless-like ubiquitin-conjugating enzyme
  • BLU
  • E2 ubiquitin-conjugating enzyme N
  • Ubc13
  • UbcH13
  • Ubiquitin carrier protein N
  • Ubiquitin-protein ligase N
Gene NameUBE2N
OrganismHuman
Amino acid sequence
>lcl|BSEQ0013924|Ubiquitin-conjugating enzyme E2 N
MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPE
EYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDP
LANDVAEQWKTNEAQAIETARAWTRLYAMNNI
Number of residues152
Molecular Weight17137.625
Theoretical pINot Available
GO Classification
Functions
  • ubiquitin conjugating enzyme activity
  • ubiquitin binding
  • ATP binding
  • ubiquitin protein ligase binding
  • ubiquitin protein ligase activity
  • ubiquitin-protein transferase activity
  • poly(A) RNA binding
Processes
  • positive regulation of NF-kappaB transcription factor activity
  • ubiquitin-dependent protein catabolic process
  • MyD88-dependent toll-like receptor signaling pathway
  • toll-like receptor 10 signaling pathway
  • cellular protein modification process
  • toll-like receptor 2 signaling pathway
  • double-strand break repair
  • DNA double-strand break processing
  • toll-like receptor 4 signaling pathway
  • double-strand break repair via homologous recombination
  • histone ubiquitination
  • positive regulation of histone modification
  • toll-like receptor 5 signaling pathway
  • innate immune response
  • positive regulation of ubiquitin-protein transferase activity
  • toll-like receptor 9 signaling pathway
  • Fc-epsilon receptor signaling pathway
  • postreplication repair
  • toll-like receptor signaling pathway
  • stimulatory C-type lectin receptor signaling pathway
  • protein K63-linked ubiquitination
  • proteolysis
  • toll-like receptor TLR1
  • cytokine-mediated signaling pathway
  • regulation of DNA repair
  • toll-like receptor TLR6
  • double-strand break repair via nonhomologous end joining
  • regulation of histone ubiquitination
  • DNA repair
  • global genome nucleotide-excision repair
  • protein ubiquitination
  • T cell receptor signaling pathway
  • positive regulation of DNA repair
  • nucleotide-excision repair
  • nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway
  • positive regulation of I-kappaB kinase/NF-kappaB signaling
  • nucleotide-binding oligomerization domain containing signaling pathway
Components
  • extracellular exosome
  • nucleolus
  • nucleoplasm
  • UBC13-MMS2 complex
  • UBC13-UEV1A complex
  • cytoplasm
  • protein complex
  • nucleus
  • ubiquitin ligase complex
  • cytosol
General FunctionUbiquitin-protein transferase activity
Specific FunctionThe UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDP61088
UniProtKB Entry NameUBE2N_HUMAN
Cellular LocationNucleus
Gene sequence
>lcl|BSEQ0013925|Ubiquitin-conjugating enzyme E2 N (UBE2N)
ATGGCCGGGCTGCCCCGCAGGATCATCAAGGAAACCCAGCGTTTGCTGGCAGAACCAGTT
CCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCCGTTATTTTCATGTGGTCATTGCT
GGCCCTCAGGATTCCCCCTTTGAGGGAGGGACTTTTAAACTTGAACTATTCCTTCCAGAA
GAATACCCAATGGCAGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTA
GACAAGTTGGGAAGAATATGTTTAGATATTTTGAAAGATAAGTGGTCCCCAGCACTGCAG
ATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAGTGCTCCCAATCCAGATGATCCA
TTAGCAAATGATGTAGCGGAGCAGTGGAAGACCAACGAAGCCCAAGCCATAGAAACAGCT
AGAGCATGGACTAGGCTATATGCCATGAATAATATTTAA
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:12492
Chromosome Location12
LocusNot Available
References
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