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NameHypoxia-inducible factor 1-alpha
Synonyms
  • ARNT-interacting protein
  • Basic-helix-loop-helix-PAS protein MOP1
  • BHLHE78
  • Class E basic helix-loop-helix protein 78
  • HIF-1-alpha
  • Member of PAS protein 1
  • MOP1
  • PAS domain-containing protein 8
  • PASD8
Gene NameHIF1A
OrganismHuman
Amino acid sequence
>lcl|BSEQ0004663|Hypoxia-inducible factor 1-alpha
MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVM
RLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYM
GLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGR
TMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSK
TFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQV
TTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECV
LKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTET
DDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEP
NPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLF
AEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVT
VFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYR
DTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKR
KMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLAC
RLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN
Number of residues826
Molecular Weight92669.595
Theoretical pI4.97
GO Classification
Functions
  • protein heterodimerization activity
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
  • transcription factor activity, RNA polymerase II transcription factor binding
  • protein kinase binding
  • transcription factor binding
  • ubiquitin protein ligase binding
  • nuclear hormone receptor binding
  • enzyme binding
  • sequence-specific DNA binding
  • transcription factor activity, transcription factor binding
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
  • histone acetyltransferase binding
  • Hsp90 protein binding
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
  • transcription factor activity, sequence-specific DNA binding
Processes
  • positive regulation of receptor biosynthetic process
  • connective tissue replacement involved in inflammatory response wound healing
  • Notch signaling pathway
  • positive regulation of pri-miRNA transcription from RNA polymerase II promoter
  • lactate metabolic process
  • positive regulation of epithelial cell migration
  • positive regulation of vascular endothelial growth factor receptor signaling pathway
  • regulation of aerobic respiration
  • angiogenesis
  • outflow tract morphogenesis
  • signal transduction
  • visual learning
  • embryonic hemopoiesis
  • heart looping
  • cellular response to interleukin-1
  • positive regulation of glycolytic process
  • positive regulation of hormone biosynthetic process
  • mRNA transcription from RNA polymerase II promoter
  • cartilage development
  • digestive tract morphogenesis
  • hypoxia-inducible factor-1alpha signaling pathway
  • intestinal epithelial cell maturation
  • positive regulation of chemokine-mediated signaling pathway
  • positive regulation of erythrocyte differentiation
  • positive regulation of neuroblast proliferation
  • regulation of transcription from RNA polymerase II promoter in response to oxidative stress
  • positive regulation of nitric-oxide synthase activity
  • negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
  • lactation
  • B-1 B cell homeostasis
  • retina vasculature development in camera-type eye
  • dopaminergic neuron differentiation
  • positive regulation of mitophagy
  • cellular iron ion homeostasis
  • cardiac ventricle morphogenesis
  • elastin metabolic process
  • cellular response to hypoxia
  • negative regulation of thymocyte apoptotic process
  • glucose homeostasis
  • response to muscle activity
  • embryonic placenta development
  • epithelial cell differentiation involved in mammary gland alveolus development
  • positive regulation of angiogenesis
  • positive regulation of insulin secretion involved in cellular response to glucose stimulus
  • negative regulation of TOR signaling
  • epithelial to mesenchymal transition
  • neural fold elevation formation
  • positive regulation of chemokine production
  • negative regulation of reactive oxygen species metabolic process
  • regulation of transcription, DNA-templated
  • negative regulation of growth
  • regulation of transforming growth factor beta2 production
  • positive regulation of vascular endothelial growth factor production
  • negative regulation of mesenchymal cell apoptotic process
  • positive regulation of transcription, DNA-templated
  • collagen metabolic process
  • negative regulation of bone mineralization
  • vascular endothelial growth factor production
  • neural crest cell migration
  • hemoglobin biosynthetic process
  • positive regulation of transcription from RNA polymerase II promoter
  • oxygen homeostasis
  • muscle cell cellular homeostasis
  • positive regulation of endothelial cell proliferation
  • axon transport of mitochondrion
  • response to hypoxia
  • regulation of gene expression
  • regulation of transcription from RNA polymerase II promoter in response to hypoxia
  • cerebral cortex development
  • positive regulation of transcription from RNA polymerase II promoter in response to hypoxia
Components
  • nuclear speck
  • cytoplasm
  • nucleus
  • transcription factor complex
  • motile cilium
  • RNA polymerase II transcription factor complex
  • cytosol
  • nucleoplasm
General FunctionUbiquitin protein ligase binding
Specific FunctionFunctions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDQ16665
UniProtKB Entry NameHIF1A_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0021673|Hypoxia-inducible factor 1-alpha (HIF1A)
ATGAGCTCCCAATGTCGGAGTTTGGAAAACAAATTTGTCTTTTTAAAAGAAGGTCTAGGA
AACTCAAAACCTGAAGAATTGGAAGAAATCAGAATAGAAAATGGTAGGATAAGTTCTGAA
CGTCGAAAAGAAAAGTCTCGAGATGCAGCCAGATCTCGGCGAAGTAAAGAATCTGAAGTT
TTTTATGAGCTTGCTCATCAGTTGCCACTTCCACATAATGTGAGTTCGCATCTTGATAAG
GCCTCTGTGATGAGGCTTACCATCAGCTATTTGCGTGTGAGGAAACTTCTGGATGCTGGT
GATTTGGATATTGAAGATGACATGAAAGCACAGATGAATTGCTTTTATTTGAAAGCCTTG
GATGGTTTTGTTATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAATGTG
AACAAATACATGGGATTAACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACT
CATCCATGTGACCATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCTTGTGAAA
AAGGGTAAAGAACAAAACACACAGCGAAGCTTTTTTCTCAGAATGAAGTGTACCCTAACT
AGCCGAGGAAGAACTATGAACATAAAGTCTGCAACATGGAAGGTATTGCACTGCACAGGC
CACATTCACGTATATGATACCAACAGTAACCAACCTCAGTGTGGGTATAAGAAACCACCT
ATGACCTGCTTGGTGCTGATTTGTGAACCCATTCCTCACCCATCAAATATTGAAATTCCT
TTAGATAGCAAGACTTTCCTCAGTCGACACAGCCTGGATATGAAATTTTCTTATTGTGAT
GAAAGAATTACCGAATTGATGGGATATGAGCCAGAAGAACTTTTAGGCCGCTCAATTTAT
GAATATTATCATGCTTTGGACTCTGATCATCTGACCAAAACTCATCATGATATGTTTACT
AAAGGACAAGTCACCACAGGACAGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGG
GTTGAAACTCAAGCAACTGTCATATATAACACCAAGAATTCTCAACCACAGTGCATTGTA
TGTGTGAATTACGTTGTGAGTGGTATTATTCAGCACGACTTGATTTTCTCCCTTCAACAA
ACAGAATGTGTCCTTAAACCGGTTGAATCTTCAGATATGAAAATGACTCAGCTATTCACC
AAAGTTGAATCAGAAGATACAAGTAGCCTCTTTGACAAACTTAAGAAGGAACCTGATGCT
TTAACTTTGCTGGCCCCAGCCGCTGGAGACACAATCATATCTTTAGATTTTGGCAGCAAC
GACACAGAAACTGATGACCAGCAACTTGAGGAAGTACCATTATATAATGATGTAATGCTC
CCCTCACCCAACGAAAAATTACAGAATATAAATTTGGCAATGTCTCCATTACCCACCGCT
GAAACGCCAAAGCCACTTCGAAGTAGTGCTGACCCTGCACTCAATCAAGAAGTTGCATTA
AAATTAGAACCAAATCCAGAGTCACTGGAACTTTCTTTTACCATGCCCCAGATTCAGGAT
CAGACACCTAGTCCTTCCGATGGAAGCACTAGACAAAGTTCACCTGAGCCTAATAGTCCC
AGTGAATATTGTTTTTATGTGGATAGTGATATGGTCAATGAATTCAAGTTGGAATTGGTA
GAAAAACTTTTTGCTGAAGACACAGAAGCAAAGAACCCATTTTCTACTCAGGACACAGAT
TTAGACTTGGAGATGTTAGCTCCCTATATCCCAATGGATGATGACTTCCAGTTACGTTCC
TTCGATCAGTTGTCACCATTAGAAAGCAGTTCCGCAAGCCCTGAAAGCGCAAGTCCTCAA
AGCACAGTTACAGTATTCCAGCAGACTCAAATACAAGAACCTACTGCTAATGCCACCACT
ACCACTGCCACCACTGATGAATTAAAAACAGTGACAAAAGACCGTATGGAAGACATTAAA
ATATTGATTGCATCTCCATCTCCTACCCACATACATAAAGAAACTACTAGTGCCACATCA
TCACCATATAGAGATACTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAAAGGAGTC
ATAGAACAGACAGAAAAATCTCATCCAAGAAGCCCTAACGTGTTATCTGTCGCTTTGAGT
CAAAGAACTACAGTTCCTGAGGAAGAACTAAATCCAAAGATACTAGCTTTGCAGAATGCT
CAGAGAAAGCGAAAAATGGAACATGATGGTTCACTTTTTCAAGCAGTAGGAATTGGAACA
TTATTACAGCAGCCAGACGATCATGCAGCTACTACATCACTTTCTTGGAAACGTGTAAAA
GGATGCAAATCTAGTGAACAGAATGGAATGGAGCAAAAGACAATTATTTTAATACCCTCT
GATTTAGCATGTAGACTGCTGGGGCAATCAATGGATGAAAGTGGATTACCACAGCTGACC
AGTTATGATTGTGAAGTTAATGCTCCTATACAAGGCAGCAGAAACCTACTGCAGGGTGAA
GAATTACTCAGAGCTTTGGATCAAGTTAACTGA
GenBank Gene IDU22431
GeneCard IDNot Available
GenAtlas IDHIF1A
HGNC IDHGNC:4910
Chromosome Location14
LocusNot Available
References
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