NameRibonuclease H1
Synonyms
  • 3.1.26.4
  • Ribonuclease H type II
  • RNase H1
  • RNH1
Gene NameRNASEH1
OrganismHuman
Amino acid sequence
>lcl|BSEQ0013656|Ribonuclease H1
MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKK
FATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKP
SVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQ
TNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVIN
KEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED
Number of residues286
Molecular Weight32064.035
Theoretical pINot Available
GO Classification
Functions
  • nucleic acid binding
  • RNA binding
  • magnesium ion binding
  • ribonuclease activity
  • RNA-DNA hybrid ribonuclease activity
Processes
  • RNA catabolic process
  • RNA phosphodiester bond hydrolysis
  • RNA phosphodiester bond hydrolysis, endonucleolytic
  • DNA replication, removal of RNA primer
Components
  • mitochondrion
General FunctionRna-dna hybrid ribonuclease activity
Specific FunctionEndonuclease that specifically degrades the RNA of RNA-DNA hybrids (PubMed:10497183). Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II (PubMed:21700224).
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDO60930
UniProtKB Entry NameRNH1_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0013657|Ribonuclease H1 (RNASEH1)
ATGTTCTATGCCGTGAGGAGGGGCCGCAAGACCGGGGTCTTTCTGACCTGGAATGAGTGC
AGAGCACAGGTGGACCGGTTTCCTGCTGCCAGATTTAAGAAGTTTGCCACAGAGGATGAG
GCCTGGGCCTTTGTCAGGAAATCTGCAAGCCCGGAAGTTTCAGAAGGGCATGAAAATCAA
CATGGACAAGAATCGGAGGCGAAAGCCAGCAAGCGACTCCGTGAGCCACTGGATGGAGAT
GGACATGAAAGCGCAGAGCCGTATGCAAAGCACATGAAGCCGAGCGTGGAGCCGGCGCCT
CCAGTTAGCAGAGACACGTTTTCCTACATGGGAGACTTCGTCGTCGTCTACACTGATGGC
TGCTGCTCCAGTAATGGGCGTAGAAGGCCGCGAGCAGGAATCGGCGTTTACTGGGGGCCA
GGCCATCCTTTAAATGTAGGCATTAGACTTCCTGGGCGGCAGACAAACCAAAGAGCGGAA
ATTCATGCAGCCTGCAAAGCCATTGAACAAGCAAAGACTCAAAACATCAATAAACTGGTT
CTGTATACAGACAGTATGTTTACGATAAATGGTATAACTAACTGGGTTCAAGGTTGGAAG
AAAAATGGGTGGAAGACAAGTGCAGGGAAAGAGGTGATCAACAAAGAGGACTTTGTGGCA
CTGGAGAGGCTTACCCAGGGGATGGACATTCAGTGGATGCATGTTCCTGGTCATTCGGGA
TTTATAGGCAATGAAGAAGCTGACAGATTAGCCAGAGAAGGAGCTAAACAATCGGAAGAC
TGA
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:18466
Chromosome Location2
LocusNot Available
References
  1. Cerritelli SM, Crouch RJ: Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI. Genomics. 1998 Nov 1;53(3):300-7. 9799596
  2. Wu H, Lima WF, Crooke ST: Molecular cloning and expression of cDNA for human RNase H. Antisense Nucleic Acid Drug Dev. 1998 Feb;8(1):53-61. 9512096
  3. Frank P, Braunshofer-Reiter C, Poltl A, Holzmann K: Cloning, subcellular localization and functional expression of human RNase HII. Biol Chem. 1998 Dec;379(12):1407-12. 9894807
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  7. Wu H, Lima WF, Crooke ST: Properties of cloned and expressed human RNase H1. J Biol Chem. 1999 Oct 1;274(40):28270-8. 10497183
  8. Skourti-Stathaki K, Proudfoot NJ, Gromak N: Human senataxin resolves RNA/DNA hybrids formed at transcriptional pause sites to promote Xrn2-dependent termination. Mol Cell. 2011 Jun 24;42(6):794-805. doi: 10.1016/j.molcel.2011.04.026. 21700224
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