Amidophosphoribosyltransferase

• 2.4.2.14
• ATase
• Glutamine phosphoribosylpyrophosphate amidotransferase
• GPATase

>lcl|BSEQ0010962|Amidophosphoribosyltransferase
MLAEIKGLNEECGVFGIWGHEEAPQITYYGLHSLQHRGQEGAGIVATDGEKLTAHKGQGL
ITEVFQNGELSKVKGKGAIGHVRYATAGGGGYENVQPLLFRSQNNGSLALAHNGNLVNAT
QLKQQLENQGSIFQTSSDTEVLAHLIKRSGHFTLKDQIKNSLSMLKGAYAFLIMTETEMI
VALDPNGLRPLSIGMMGDAYVVASETCAFDVVGATYLREVEPGEMLIINDEGMKSERFSM
NINRSICSMEYIYFSRPDSNIDGINVHSARKNLGKMLAQESAVEADVVTGVPDSSISAAI
GYAEATGIPYELGLIKNRYVGRTFIQPSQALREQGVRMKLSAVRGVVEGKRVVMVDDSIV
RGTTSRRIVTMLREAGATEVHVKISSPPIAHPCFYGIDTSTHEELIASSHSVEEIRQEIG
ADTLSFLSVEGLLKGIGRKYDDSNCGQCLACFTGKYPTEIYQDTVLPHVKEAVLTK

• Pribosyltran (PF00156 )

>lcl|BSEQ0010963|Amidophosphoribosyltransferase (purF)
ATGCTTGCTGAAATCAAAGGCTTAAATGAAGAATGCGGCGTTTTTGGGATTTGGGGACAT
GAAGAAGCCCCGCAAATCACGTATTACGGTCTCCACAGCCTTCAGCACCGAGGACAGGAG
GGTGCTGGCATCGTAGCGACTGACGGTGAAAAGCTGACGGCTCACAAAGGCCAAGGTCTG
ATCACTGAAGTATTTCAAAACGGCGAACTCAGCAAAGTAAAGGGAAAAGGCGCTATCGGG
CACGTTCGGTACGCAACGGCTGGAGGCGGCGGATACGAAAATGTTCAGCCGCTCCTCTTC
CGTTCCCAAAACAACGGCAGCCTGGCGCTTGCTCATAACGGAAATCTTGTCAACGCCACT
CAGCTGAAGCAGCAGCTCGAAAATCAAGGGAGCATCTTTCAAACCTCTTCGGATACAGAG
GTTTTGGCTCACCTGATCAAAAGAAGCGGACACTTCACGCTGAAGGATCAAATTAAAAAC
TCGCTTTCTATGCTGAAAGGCGCCTACGCGTTCCTGATCATGACCGAAACAGAAATGATT
GTCGCACTTGATCCAAACGGGCTGAGACCGCTATCCATCGGCATGATGGGCGACGCTTAT
GTGGTCGCATCAGAAACATGCGCATTTGACGTCGTCGGCGCAACGTACCTTCGCGAGGTA
GAGCCGGGAGAAATGCTGATCATTAATGATGAAGGCATGAAATCAGAGCGTTTTTCCATG
AATATCAATCGTTCCATTTGCAGCATGGAGTACATTTATTTCTCCAGACCAGACAGCAAT
ATTGACGGTATTAATGTGCACAGTGCCCGTAAAAACCTTGGGAAAATGCTGGCTCAGGAA
TCCGCAGTTGAAGCTGACGTCGTAACCGGGGTTCCGGATTCCAGTATTTCAGCGGCGATC
GGCTATGCAGAGGCAACAGGCATTCCGTATGAGCTTGGCTTAATCAAAAACCGTTATGTT
GGCAGAACGTTTATTCAGCCGTCCCAGGCTCTGCGTGAGCAAGGCGTCAGAATGAAGCTG
TCTGCGGTGCGCGGGGTTGTAGAAGGCAAACGCGTCGTGATGGTGGATGACTCTATCGTG
CGAGGAACAACTAGCCGCCGGATTGTCACGATGCTAAGAGAAGCGGGTGCGACAGAGGTG
CATGTGAAAATCAGTTCACCGCCGATCGCTCATCCGTGCTTTTACGGCATTGACACTTCC
ACACATGAAGAACTGATCGCGTCTTCGCATTCTGTTGAAGAAATCCGTCAGGAAATCGGA
GCCGATACCCTCTCATTTTTGAGTGTGGAAGGGCTGCTGAAAGGCATCGGCAGAAAATAC
GATGACTCGAATTGCGGACAGTGTCTCGCTTGCTTTACAGGAAAATATCCGACTGAAATT
TACCAGGATACAGTGCTTCCTCACGTAAAAGAAGCAGTATTAACCAAATAA

1. Makaroff CA, Zalkin H, Switzer RL, Vollmer SJ: Cloning of the Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase gene in Escherichia coli. Nucleotide sequence determination and properties of the plasmid-encoded enzyme. J Biol Chem. 1983 Sep 10;258(17):10586-93. 6411717
2. Ebbole DJ, Zalkin H: Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis. J Biol Chem. 1987 Jun 15;262(17):8274-87. 3036807
3. Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Cordani JJ, Connerton IF, Cummings NJ, Daniel RA, Denziot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Togoni A, Tosato V, Uchiyama S, Vandebol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A: The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. 9384377
4. Barbe V, Cruveiller S, Kunst F, Lenoble P, Meurice G, Sekowska A, Vallenet D, Wang T, Moszer I, Medigue C, Danchin A: From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later. Microbiology. 2009 Jun;155(Pt 6):1758-75. doi: 10.1099/mic.0.027839-0. Epub 2009 Apr 21. 19383706
5. Wong JY, Bernlohr DA, Turnbough CL, Switzer RL: Purification and properties of glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis. Biochemistry. 1981 Sep 29;20(20):5669-74. 6794613
6. Vollmer SJ, Switzer RL, Hermodson MA, Bower SG, Zalkin H: The glutamine-utilizing site of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase. J Biol Chem. 1983 Sep 10;258(17):10582-5. 6411716
7. Mantsala P, Zalkin H: Glutamine amidotransferase function. Replacement of the active-site cysteine in glutamine phosphoribosylpyrophosphate amidotransferase by site-directed mutagenesis. J Biol Chem. 1984 Nov 25;259(22):14230-6. 6094545
8. Makaroff CA, Paluh JL, Zalkin H: Mutagenesis of ligands to the [4 Fe-4S] center of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase. J Biol Chem. 1986 Aug 25;261(24):11416-23. 3090047
9. Smith JL, Zaluzec EJ, Wery JP, Niu L, Switzer RL, Zalkin H, Satow Y: Structure of the allosteric regulatory enzyme of purine biosynthesis. Science. 1994 Jun 3;264(5164):1427-33. 8197456
10. Chen S, Tomchick DR, Wolle D, Hu P, Smith JL, Switzer RL, Zalkin H: Mechanism of the synergistic end-product regulation of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase by nucleotides. Biochemistry. 1997 Sep 2;36(35):10718-26. 9271502