Tryptophan--tRNA ligase

NameTryptophan--tRNA ligase
Synonyms
  • 6.1.1.2
  • TrpRS
  • Tryptophanyl-tRNA synthetase
Gene NametrpS
OrganismGeobacillus stearothermophilus
Amino acid sequence
>lcl|BSEQ0010937|Tryptophan--tRNA ligase
MKTIFSGIQPSGVITIGNYIGALRQFVELQHEYNCYFCIVDQHAITVWQDPHELRQNIRR
LAAKYLAVGIDPTQATLFIQSEVPAHAQAAWMLQCIVYIGELERMTQFKEKSAGKEAVSA
GLLTYPPLMAADILLYNTDIVPVGEDQKQHIELTRDLAERFNKRYGELFTIPEARIPKVG
ARIMSLVDPTKKMSKSDPNPKAYITLLDDAKTIEKKIKSAVTDSEGTIRYDKEAKPGISN
LLNIYSTLSGQSIEELERQYEGKGYGVFKADLAQVVIETLRPIQERYHHWMESEELDRVL
DEGAEKANRVASEMVRKMEQAMGLGRRR
Number of residues328
Molecular Weight37192.385
Theoretical pI6.64
GO Classification
Functions
  • ATP binding
  • tryptophan-tRNA ligase activity
Processes
  • tryptophanyl-tRNA aminoacylation
Components
  • cytoplasm
General FunctionTryptophan-trna ligase activity
Specific FunctionNot Available
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID143784
UniProtKB IDP00953
UniProtKB Entry NameSYW_GEOSE
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0002666|987 bp
ATGAAAACCATTTTTTCCGGCATTCAGCCAAGCGGCGTCATCACCATTGGCAACTATATT
GGGGCGCTGCGGCAGTTTGTCGAGCTGCAGCATGAATACAACTGCTATTTTTGCATCGTT
GACCAACACGCCATTACCGTTTGGCAAGACCCACACGAACTGCGGCAAAACATCCGCCGC
CTCGCCGCAAAGTATTTGGCCGTCGGCATCGACCCGACGCAAGCGACGTTGTTCATCCAG
TCAGAAGTGCCGGCGCACGCTCAAGCCGCTTGGATGCTGCAGTGCATCGTTTATATCGGC
GAACTTGAGCGGATGACGCAATTTAAAGAAAAATCGGCCGGCAAAGAGGCGGTCAGCGCC
GGGCTGCTCACGTACCCGCCGCTGATGGCCGCTGACATTTTGCTTTATAACACCGACATC
GTCCCGGTCGGCGAAGACCAAAAGCAGCATATCGAATTGACGCGGGATCTGGCCGAGCGC
TTCAACAAACGGTATGGCGAGCTGTTTACGATCCCTGAGGCGCGCATTCCGAAAGTCGGC
GCCCGCATCATGTCGCTTGTCGACCCGACGAAAAAAATGAGCAAATCCGACCCCAACCCG
AAAGCGTACATCACACTGCTTGACGATGCGAAAACGATCGAGAAGAAGATCAAAAGCGCG
GTCACCGACTCGGAAGGAACGATTCGCTACGACAAAGAAGCGAAGCCAGGCATTTCGAAC
TTGCTCAATATTTATTCGACCCTATCCGGTCAATCGATCGAGGAGTTGGAGCGGCAATAC
GAAGGAAAAGGATACGGCGTCTTCAAAGCAGACCTCGCTCAAGTCGTCATCGAAACGCTT
CGACCGATTCAAGAGCGGTATCACCATTGGATGGAAAGCGAGGAGCTTGACCGCGTGTTG
GATGAAGGGGCGGAAAAAGCAAATCGGGTTGCATCGGAAATGGTGCGAAAAATGGAGCAA
GCCATGGGGCTCGGGCGGCGGCGGTAA
GenBank Gene IDM14742
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Barstow DA, Sharman AF, Atkinson T, Minton NP: Cloning and complete nucleotide sequence of the Bacillus stearothermophilus tryptophanyl tRNA synthetase gene. Gene. 1986;46(1):37-45. 3026925
  2. Winter GP, Hartley BS: The amino acid sequence of tryptophanyl tRNA synthetase from Bacillus stearothermophilus. FEBS Lett. 1977 Aug 15;80(2):340-2. 891985
  3. Doublie S, Bricogne G, Gilmore C, Carter CW Jr: Tryptophanyl-tRNA synthetase crystal structure reveals an unexpected homology to tyrosyl-tRNA synthetase. Structure. 1995 Jan 15;3(1):17-31. 7743129
  4. Ilyin VA, Temple B, Hu M, Li G, Yin Y, Vachette P, Carter CW Jr: 2.9 A crystal structure of ligand-free tryptophanyl-tRNA synthetase: domain movements fragment the adenine nucleotide binding site. Protein Sci. 2000 Feb;9(2):218-31. 10716174