N-acetylneuraminate lyase

NameN-acetylneuraminate lyase
Synonyms
  • 4.1.3.3
  • N-acetylneuraminate pyruvate-lyase
  • N-acetylneuraminic acid aldolase
  • NAL
  • NALase
  • Sialate lyase
  • Sialic acid aldolase
  • Sialic acid lyase
Gene NamenanA
OrganismShigella flexneri
Amino acid sequence
>lcl|BSEQ0011174|N-acetylneuraminate lyase
MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYVGGSTGEAFVQSLSERE
QVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDAVSAVTPFYYPFSFEEHCD
HYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPGVGALKQTSGDLYQMEQIRRE
HPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQGIVKALKEGDIQTAQKLQTECN
KVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGPVDEKYLPELKALAQQLMQERG
Number of residues297
Molecular Weight32593.23
Theoretical pI5.69
GO Classification
Functions
  • N-acetylneuraminate lyase activity
Processes
  • N-acetylneuraminate catabolic process
  • carbohydrate metabolic process
Components
  • cytoplasm
General FunctionN-acetylneuraminate lyase activity
Specific FunctionCatalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID24053696
UniProtKB IDP0A6L6
UniProtKB Entry NameNANA_SHIFL
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0011175|N-acetylneuraminate lyase (nanA)
ATGGCAACGAATTTACGTGGCGTAATGGCTGCACTCCTGACTCCTTTTGATCAACAACAA
GCACTGGATAAAGCGAGTCTGCGCCGCCTGGTTCAGTTCAATATTCAGCAGGGCATCGAC
GGTTTATACGTGGGTGGTTCGACCGGCGAAGCCTTTGTACAAAGCCTTTCCGAGCGTGAA
CAGGTACTGGAAATCGTCGCCGAAGAGGCGAAAGGTAAGATTAAACTCATCGCCCACGTC
GGTTGCGTCAGCACCGCCGAAAGCCAACAACTTGCGGCATCGGCTAAACGTTATGGCTTC
GATGCCGTCTCCGCCGTCACGCCGTTCTACTATCCTTTCAGCTTTGAAGAACACTGCGAT
CACTATCGGGCAATTATTGATTCAGCGGATGGTTTGCCGATGGTGGTGTACAACATTCCA
GCCCTGAGTGGGGTAAAACTGACCCTGGATCAGATCAACACCCTGGTTACGTTGCCTGGC
GTCGGTGCGCTGAAACAGACTTCTGGCGATCTCTATCAGATGGAACAGATCCGTCGTGAA
CATCCTGATCTGGTGCTCTATAACGGTTACGACGAAATCTTCGCTTCCGGCTTGCTGGCA
GGCGCTGATGGTGGTATCGGCAGTACTTACAACATCATGGGCTGGCGATATCAGGGGATC
GTGAAGGCGCTGAAAGAAGGCGATATCCAGACCGCGCAGAAACTGCAAACTGAGTGCAAC
AAAGTCATTGATTTACTGATCAAAACGGGCGTATTCCGCGGCCTGAAAACTGTCCTGCAT
TATATGGATGTCGTTTCTGTGCCGCTGTGCCGCAAACCGTTTGGACCGGTAGATGAAAAA
TATCTGCCAGAACTGAAGGCGCTGGCCCAGCAGTTGATGCAAGAGCGCGGGTGA
GenBank Gene IDAE005674
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Jin Q, Yuan Z, Xu J, Wang Y, Shen Y, Lu W, Wang J, Liu H, Yang J, Yang F, Zhang X, Zhang J, Yang G, Wu H, Qu D, Dong J, Sun L, Xue Y, Zhao A, Gao Y, Zhu J, Kan B, Ding K, Chen S, Cheng H, Yao Z, He B, Chen R, Ma D, Qiang B, Wen Y, Hou Y, Yu J: Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157. Nucleic Acids Res. 2002 Oct 15;30(20):4432-41. 12384590
  2. Wei J, Goldberg MB, Burland V, Venkatesan MM, Deng W, Fournier G, Mayhew GF, Plunkett G 3rd, Rose DJ, Darling A, Mau B, Perna NT, Payne SM, Runyen-Janecky LJ, Zhou S, Schwartz DC, Blattner FR: Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T. Infect Immun. 2003 May;71(5):2775-86. 12704152