Hypoxanthine phosphoribosyltransferase

NameHypoxanthine phosphoribosyltransferase
Synonyms
  • 2.4.2.8
  • HPRT
Gene Namehpt
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0011507|Hypoxanthine phosphoribosyltransferase
MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRGSFMFMADLCREVQVSH
EVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDIIDSGNTLSKVREILSLREP
KSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDYAQRYRHLPYIGKVILLDE
Number of residues178
Molecular Weight20115.025
Theoretical pI4.82
GO Classification
Functions
  • magnesium ion binding
  • guanine phosphoribosyltransferase activity
  • nucleotide binding
  • hypoxanthine phosphoribosyltransferase activity
Processes
  • adenine salvage
  • GMP salvage
  • guanine salvage
  • hypoxanthine metabolic process
  • IMP salvage
Components
  • cytosol
General FunctionNucleotide binding
Specific FunctionActs preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDP0A9M2
UniProtKB Entry NameHPRT_ECOLI
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0011508|Hypoxanthine phosphoribosyltransferase (hpt)
ATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAA
CTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGT
CTGCTGCGTGGCTCATTTATGTTTATGGCGGACCTGTGCCGTGAAGTTCAGGTATCTCAT
GAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTG
AAAATCCTCAAAGATCTGGATGAAGATATCCGTGGCAAGGACGTGCTGATTGTTGAAGAT
ATCATCGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCG
AAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCG
GTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTAC
GCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAA
GenBank Gene IDU00096
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Fujita N, Mori H, Yura T, Ishihama A: Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region. Nucleic Acids Res. 1994 May 11;22(9):1637-9. 8202364
  2. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. 9278503
  3. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. 16738553
  4. Guddat LW, Vos S, Martin JL, Keough DT, de Jersey J: Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase. Protein Sci. 2002 Jul;11(7):1626-38. 12070315