Glycerol uptake facilitator protein

NameGlycerol uptake facilitator protein
Synonyms
  • Aquaglyceroporin
Gene NameglpF
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0016653|Glycerol uptake facilitator protein
MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISVIWGLGVAMAIYLTA
GVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAALVYGLYYNLFFDFEQTHH
IVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILMGLILALTDDGNGVPRGPLAP
LLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAGWGNVAFTGGRDIPYFLVPLFGP
IVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSEQKASL
Number of residues281
Molecular Weight29779.71
Theoretical pI6.66
GO Classification
Functions
  • glycerol transmembrane transporter activity
  • metal ion binding
  • glycerol channel activity
  • water channel activity
Processes
  • cellular water homeostasis
  • glycerol transport
  • cellular response to mercury ion
  • ion transmembrane transport
  • water transport
Components
  • plasma membrane
  • integral component of plasma membrane
General FunctionWater channel activity
Specific FunctionTransporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.
Pfam Domain Function
Transmembrane Regions6-34 40-60 85-108 144-169 178-194 232-254
GenBank Protein IDNot Available
UniProtKB IDP0AER0
UniProtKB Entry NameGLPF_ECOLI
Cellular LocationCell inner membrane
Gene sequence
>lcl|BSEQ0016654|Glycerol uptake facilitator protein (glpF)
ATGAGTCAAACATCAACCTTGAAAGGCCAGTGCATTGCTGAATTCCTCGGTACCGGGTTG
TTGATTTTCTTCGGTGTGGGTTGCGTTGCAGCACTAAAAGTCGCTGGTGCGTCTTTTGGT
CAGTGGGAAATCAGTGTCATTTGGGGACTGGGGGTGGCAATGGCCATCTACCTGACCGCA
GGGGTTTCCGGCGCGCATCTTAATCCCGCTGTTACCATTGCATTGTGGCTGTTTGCCTGT
TTCGACAAGCGCAAAGTTATTCCTTTTATCGTTTCACAAGTTGCCGGCGCTTTCTGTGCT
GCGGCTTTAGTTTACGGGCTTTACTACAATTTATTTTTCGACTTCGAGCAGACTCATCAC
ATTGTTCGCGGCAGCGTTGAAAGTGTTGATCTGGCTGGCACTTTCTCTACTTACCCTAAT
CCTCATATCAATTTTGTGCAGGCTTTCGCAGTTGAGATGGTGATTACCGCTATTCTGATG
GGGCTGATCCTGGCGTTAACGGACGATGGCAACGGTGTACCACGCGGCCCTTTGGCTCCC
TTGCTGATTGGTCTACTGATTGCGGTCATTGGCGCATCTATGGGCCCATTGACAGGTTTT
GCCATGAACCCAGCGCGTGACTTCGGTCCGAAAGTCTTTGCCTGGCTGGCGGGCTGGGGC
AATGTCGCCTTTACCGGCGGCAGAGACATTCCTTACTTCCTGGTGCCGCTTTTCGGCCCT
ATCGTTGGCGCGATTGTAGGTGCATTTGCCTACCGCAAACTGATTGGTCGCCATTTGCCT
TGCGATATCTGTGTTGTGGAAGAAAAGGAAACCACAACTCCTTCAGAACAAAAAGCTTCG
CTGTAA
GenBank Gene IDX15054
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Muramatsu S, Mizuno T: Nucleotide sequence of the region encompassing the glpKF operon and its upstream region containing a bent DNA sequence of Escherichia coli. Nucleic Acids Res. 1989 Jun 12;17(11):4378. 2544860
  2. Weissenborn DL, Wittekindt N, Larson TJ: Structure and regulation of the glpFK operon encoding glycerol diffusion facilitator and glycerol kinase of Escherichia coli K-12. J Biol Chem. 1992 Mar 25;267(9):6122-31. 1372899
  3. Plunkett G 3rd, Burland V, Daniels DL, Blattner FR: Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 1993 Jul 25;21(15):3391-8. 8346018
  4. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. 9278503
  5. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. 16738553
  6. Heller KB, Lin EC, Wilson TH: Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli. J Bacteriol. 1980 Oct;144(1):274-8. 6998951
  7. Borgnia MJ, Agre P: Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2888-93. Epub 2001 Feb 20. 11226336
  8. Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G: Global topology analysis of the Escherichia coli inner membrane proteome. Science. 2005 May 27;308(5726):1321-3. 15919996
  9. Fu D, Libson A, Miercke LJ, Weitzman C, Nollert P, Krucinski J, Stroud RM: Structure of a glycerol-conducting channel and the basis for its selectivity. Science. 2000 Oct 20;290(5491):481-6. 11039922
  10. Unger VM: Fraternal twins: AQP1 and GlpF. Nat Struct Biol. 2000 Dec;7(12):1082-4. 11101882
  11. Tajkhorshid E, Nollert P, Jensen MO, Miercke LJ, O'Connell J, Stroud RM, Schulten K: Control of the selectivity of the aquaporin water channel family by global orientational tuning. Science. 2002 Apr 19;296(5567):525-30. 11964478