Pyridoxine/pyridoxamine 5'-phosphate oxidase

NamePyridoxine/pyridoxamine 5'-phosphate oxidase
Synonyms
  • 1.4.3.5
  • PNP/PMP oxidase
  • PNPOx
  • Pyridoxal 5'-phosphate synthase
Gene NamepdxH
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0016666|Pyridoxine/pyridoxamine 5'-phosphate oxidase
MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACEAKLADPTAMVVATVDE
HGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLLFPWHTLERQVMVIGKAER
LSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKFLELKQKFQQGEVPLPSFWGG
FRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
Number of residues218
Molecular Weight25544.975
Theoretical pI9.6
GO Classification
Functions
  • FMN binding
  • oxidoreductase activity
  • pyridoxamine-phosphate oxidase activity
Processes
  • oxidation-reduction process
  • pyridoxine biosynthetic process
  • pyridoxal 5'-phosphate salvage
Components
  • cytosol
General FunctionPyridoxamine-phosphate oxidase activity
Specific FunctionCatalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDP0AFI7
UniProtKB Entry NamePDXH_ECOLI
Cellular LocationNot Available
Gene sequence
>lcl|BSEQ0016667|Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH)
ATGTCTGATAACGACGAATTGCAGCAAATCGCGCATCTGCGCCGTGAATACACCAAAGGC
GGGTTACGCCGCCGCGATCTTCCCGCCGATCCATTAACCCTTTTTGAACGTTGGCTCTCT
CAGGCTTGTGAAGCCAAACTGGCGGACCCTACCGCGATGGTGGTCGCTACCGTGGATGAA
CATGGTCAGCCTTATCAGCGCATCGTTTTACTCAAACATTACGACGAAAAAGGCATGGTG
TTTTACACCAACCTCGGCAGCCGTAAAGCACATCAAATCGAAAATAATCCGCGCGTTAGC
CTGCTGTTCCCGTGGCATACCCTTGAGCGCCAGGTGATGGTGATCGGTAAAGCAGAACGA
CTTTCGACTCTCGAAGTGATGAAATATTTTCATAGCCGCCCGCGTGATAGCCAGATTGGT
GCATGGGTTTCGAAGCAGTCCAGTCGCATTTCTGCCCGCGGTATCCTTGAAAGTAAATTC
CTGGAGCTGAAGCAGAAGTTTCAACAGGGCGAAGTGCCATTGCCGAGCTTTTGGGGCGGT
TTTCGCGTCAGCCTTGAACAGATTGAGTTCTGGCAGGGTGGTGAGCATCGCCTGCATGAC
CGCTTTTTGTACCAGCGTGAAAATGATGCGTGGAAGATTGATCGTCTTGCACCCTGA
GenBank Gene IDM92351
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Lam HM, Winkler ME: Characterization of the complex pdxH-tyrS operon of Escherichia coli K-12 and pleiotropic phenotypes caused by pdxH insertion mutations. J Bacteriol. 1992 Oct;174(19):6033-45. 1356963
  2. Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.: A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):363-77. 9097039
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. 9278503
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. 16738553
  5. Zhao G, Winkler ME: Kinetic limitation and cellular amount of pyridoxine (pyridoxamine) 5'-phosphate oxidase of Escherichia coli K-12. J Bacteriol. 1995 Feb;177(4):883-91. 7860596
  6. Di Salvo M, Yang E, Zhao G, Winkler ME, Schirch V: Expression, purification, and characterization of recombinant Escherichia coli pyridoxine 5'-phosphate oxidase. Protein Expr Purif. 1998 Aug;13(3):349-56. 9693059
  7. Safo MK, Mathews I, Musayev FN, di Salvo ML, Thiel DJ, Abraham DJ, Schirch V: X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with FMN at 1.8 A resolution. Structure. 2000 Jul 15;8(7):751-62. 10903950
  8. Safo MK, Musayev FN, di Salvo ML, Schirch V: X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with pyridoxal 5'-phosphate at 2.0 A resolution. J Mol Biol. 2001 Jul 20;310(4):817-26. 11453690
  9. di Salvo ML, Ko TP, Musayev FN, Raboni S, Schirch V, Safo MK: Active site structure and stereospecificity of Escherichia coli pyridoxine-5'-phosphate oxidase. J Mol Biol. 2002 Jan 18;315(3):385-97. 11786019
  10. Safo MK, Musayev FN, Schirch V: Structure of Escherichia coli pyridoxine 5'-phosphate oxidase in a tetragonal crystal form: insights into the mechanistic pathway of the enzyme. Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):599-604. Epub 2005 Apr 20. 15858270