Adenine DNA glycosylase

NameAdenine DNA glycosylase
Synonyms
  • 3.2.2.-
  • A/G-specific adenine glycosylase
  • micA
Gene NamemutY
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0011939|Adenine DNA glycosylase
MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQVATVIPYFERFMARFP
TVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGGKFPETFEEVAALPGVGRS
TAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEVENKLWSLSEQVTPAVGVERF
NQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWALYPGKKPKQTLPERTGYFLLLQH
EDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQRQIAADNLTQLTAFRHTFSHFHLD
IVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLAAPVERLLQQLRTGAPV
Number of residues350
Molecular Weight39148.835
Theoretical pI8.46
GO Classification
Functions
  • hydrolase activity
  • DNA binding
  • metal ion binding
  • DNA N-glycosylase activity
  • 4 iron, 4 sulfur cluster binding
Processes
  • base-excision repair
General FunctionMetal ion binding
Specific FunctionAdenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine: 7,8-dihydro-8-oxoguanine (8-oxo-dGTP).
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDP17802
UniProtKB Entry NameMUTY_ECOLI
Cellular LocationCytoplasmic
Gene sequence
>lcl|BSEQ0011940|Adenine DNA glycosylase (mutY)
ATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAA
ACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTG
CAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCG
ACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTT
GGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCACAACAAGTGGCGACCTTACAC
GGCGGTAAATTCCCGGAAACCTTTGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCC
ACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAAC
GTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGGCCTGGGAAAAAAGAGGTC
GAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTT
AATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTCG
CTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCG
GGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCAC
GAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGT
TTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCT
GCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGAT
ATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCG
CTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTG
TTACAGCAGTTACGCACTGGCGCGCCGGTTTAG
GenBank Gene IDX52391
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Michaels ML, Pham L, Nghiem Y, Cruz C, Miller JH: MutY, an adenine glycosylase active on G-A mispairs, has homology to endonuclease III. Nucleic Acids Res. 1990 Jul 11;18(13):3841-5. 2197596
  2. Tsai-Wu JJ, Radicella JP, Lu AL: Nucleotide sequence of the Escherichia coli micA gene required for A/G-specific mismatch repair: identity of micA and mutY. J Bacteriol. 1991 Mar;173(6):1902-10. 2001994
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. 9278503
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. 16738553
  5. Guan Y, Manuel RC, Arvai AS, Parikh SS, Mol CD, Miller JH, Lloyd S, Tainer JA: MutY catalytic core, mutant and bound adenine structures define specificity for DNA repair enzyme superfamily. Nat Struct Biol. 1998 Dec;5(12):1058-64. 9846876