Arachidonate 12-lipoxygenase, 12S-type

NameArachidonate 12-lipoxygenase, 12S-type
Synonyms
  • 1.13.11.31
  • 12LO
  • 12S-LOX
  • Lipoxin synthase 12-LO
  • LOG12
  • Platelet-type lipoxygenase 12
Gene NameALOX12
OrganismHuman
Amino acid sequence
>lcl|BSEQ0017198|Arachidonate 12-lipoxygenase, 12S-type
MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFDHDVAEDLGLL
QFVRLRKHHWLVDDAWFCDRITVQGPGACAEVAFPCYRWVQGEDILSLPEGTARLPGDNA
LDMFQKHREKELKDRQQIYCWATWKEGLPLTIAADRKDDLPPNMRFHEEKRLDFEWTLKA
GALEMALKRVYTLLSSWNCLEDFDQIFWGQKSALAEKVRQCWQDDELFSYQFLNGANPML
LRRSTSLPSRLVLPSGMEELQAQLEKELQNGSLFEADFILLDGIPANVIRGEKQYLAAPL
VMLKMEPNGKLQPMVIQIQPPNPSSPTPTLFLPSDPPLAWLLAKSWVRNSDFQLHEIQYH
LLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINTRARTQLISDGGIFDKAVST
GGGGHVQLLRRAAAQLTYCSLCPPDDLADRGLLGLPGALYAHDALRLWEIIARYVEGIVH
LFYQRDDIVKGDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQH
AAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRR
QPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENS
VTI
Number of residues663
Molecular Weight75693.38
Theoretical pI6.15
GO Classification
Functions
  • oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
  • linoleate 13S-lipoxygenase activity
  • arachidonate 12-lipoxygenase activity
  • hepoxilin A3 synthase activity
  • hepoxilin-epoxide hydrolase activity
  • iron ion binding
Processes
  • negative regulation of platelet aggregation
  • lipoxin metabolic process
  • fatty acid oxidation
  • establishment of skin barrier
  • lipoxygenase pathway
  • reactive oxygen species metabolic process
  • positive regulation of gene expression
  • small molecule metabolic process
  • superoxide anion generation
  • aging
  • cellular response to lipid
  • hepoxilin metabolic process
  • positive regulation of cell migration
  • leukotriene A4 metabolic process
  • hepoxilin biosynthetic process
  • negative regulation of apoptotic process
  • lipoxin B4 biosynthetic process
  • lipoxin A4 biosynthetic process
  • positive regulation of cell growth
  • positive regulation of vasodilation
  • positive regulation of cell proliferation
  • positive regulation of endothelial cell differentiation
  • movement of cell or subcellular component
  • positive regulation of cell adhesion
  • positive regulation of endothelial cell migration
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
  • negative regulation of muscle cell apoptotic process
  • positive regulation of smooth muscle cell proliferation
  • linoleic acid metabolic process
  • positive regulation of angiogenesis
  • positive regulation of mitochondrial depolarization
  • arachidonic acid metabolic process
Components
  • cytosol
  • extracellular exosome
  • sarcolemma
  • cytoplasm
  • membrane
General FunctionOxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
Specific FunctionNon-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID189774
UniProtKB IDP18054
UniProtKB Entry NameLOX12_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0017199|Arachidonate 12-lipoxygenase, 12S-type (ALOX12)
ATGGGCCGCTACCGCATCCGCGTGGCCACCGGGGCCTGGCTCTTCTCCGGGTCGTACAAC
CGCGTGCAGCTTTGGCTGGTCGGGACGCGCGGGGAGGCGGAGCTGGAGCTGCAGCTGCGG
CCCGCGCGGGGCGAGGAGGAGGAGTTTGATCATGACGTTGCAGAGGACTTGGGGCTCCTG
CAGTTCGTGAGGCTGCGCAAGCACCACTGGCTGGTGGACGACGCGTGGTTCTGCGACCGC
ATCACGGTGCAGGGCCCTGGAGCCTGCGCGGAGGTGGCCTTCCCGTGCTACCGCTGGGTG
CAGGGCGAGGACATCCTGAGCCTGCCCGAGGGCACCGCCCGCCTGCCAGGAGACAATGCT
TTGGACATGTTCCAGAAGCATCGAGAGAAGGAACTGAAAGACAGACAGCAGATCTACTGC
TGGGCCACCTGGAAGGAAGGGTTACCCCTGACCATCGCTGCAGACCGTAAGGATGATCTA
CCTCCAAATATGAGATTCCATGAGGAGAAGAGGCTGGACTTTGAATGGACACTGAAGGCA
GGGGCTCTGGAGATGGCCCTCAAACGTGTTTACACCCTCCTGAGCTCCTGGAACTGCCTA
GAAGACTTTGATCAGATCTTCTGGGGCCAGAAGAGTGCCCTGGCTGAGAAGGTTCGCCAG
TGCTGGCAGGATGATGAGTTGTTCAGCTACCAGTTCCTCAATGGTGCCAACCCCATGCTG
TTGAGACGCTCGACCTCTCTGCCCTCCAGGCTAGTGCTGCCCTCGGGGATGGAAGAGCTT
CAGGCTCAACTGGAGAAAGAACTTCAGAATGGTTCCCTGTTTGAAGCTGACTTCATCCTT
CTGGATGGAATTCCAGCCAACGTGATCCGAGGAGAGAAGCAATACCTGGCTGCCCCCCTC
GTTATGCTGAAGATGGAGCCCAATGGGAAGCTGCAGCCCATGGTCATCCAGATTCAGCCT
CCCAACCCCAGCTCTCCAACCCCAACACTGTTCCTGCCCTCAGACCCCCCACTTGCCTGG
CTCCTGGCAAAGTCCTGGGTCCGAAATTCAGATTTCCAACTGCACGAGATCCAGTATCAC
TTGCTGAACACTCACCTGGTGGCTGAGGTCATCGCTGTCGCCACCATGCGGTGCCTCCCA
GGACTGCACCCCATCTTCAAGTTCCTGATCCCCCATATCCGCTACACCATGGAAATCAAC
ACCCGGGCCCGGACCCAACTCATCTCAGATGGAGGAATTTTTGATAAGGCAGTGAGCACA
GGTGGAGGGGGCCATGTACAGTTGCTCCGTCGGGCGGCAGCTCAGCTGACCTACTGCTCC
CTCTGTCCTCCTGACGACCTGGCTGACCGGGGCCTGCTGGGACTCCCAGGTGCTCTCTAT
GCCCATGATGCTTTACGGCTCTGGGAGATCATTGCCAGGTATGTGGAGGGGATCGTCCAC
CTCTTCTACCAAAGGGATGACATAGTGAAGGGGGACCCTGAGCTGCAGGCCTGGTGTCGG
GAGATCACGGAGGTGGGGCTGTGCCAGGCCCAGGACCGAGGTTTCCCTGTCTCCTTCCAG
TCCCAGAGTCAACTCTGCCATTTCCTCACCATGTGCGTCTTCACGTGCACTGCCCAGCAT
GCCGCCATCAACCAGGGCCAGCTGGACTGGTATGCCTGGGTCCCTAATGCTCCATGCACA
ATGCGGATGCCCCCACCCACCACCAAGGAAGATGTGACGATGGCCACAGTGATGGGGTCA
CTACCTGATGTCCGGCAGGCCTGTCTTCAAATGGCCATCTCATGGCATCTGAGTCGCCGC
CAGCCAGACATGGTGCCTCTGGGGCACCACAAAGAAAAATATTTCTCAGGCCCCAAGCCC
AAAGCTGTGCTAAACCAATTCCGAACAGATTTGGAAAAGCTGGAAAAGGAGATTACAGCC
CGGAATGAGCAACTTGACTGGCCCTATGAATATCTGAAGCCCAGCTGCATAGAGAACAGT
GTCACCATCTGA
GenBank Gene IDM35418
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:429
Chromosome Location17
Locus17p13.1
References
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