Farnesyl diphosphate synthase

NameFarnesyl diphosphate synthase
Synonyms
  • (2E,6E)-farnesyl diphosphate synthase
  • 2.5.1.10
  • FPP synthase
  • Geranyltranstransferase
Gene NameispA
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0011072|Farnesyl diphosphate synthase
MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGGKRLRPFLVYATGHMFG
VSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCHVKFGEANAILAGDALQTL
AFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQALDLDAEGKHVPLDALERIHRH
KTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLAFQVQDDILDVVGDTATLGKRQG
ADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQLAEQSLDTSALEALADYIIQRNK
Number of residues299
Molecular Weight32159.22
Theoretical pI5.18
GO Classification
Functions
  • geranyltranstransferase activity
  • metal ion binding
  • dimethylallyltranstransferase activity
Processes
  • farnesyl diphosphate biosynthetic process
  • geranyl diphosphate biosynthetic process
Components
  • cytosol
General FunctionMetal ion binding
Specific FunctionNot Available
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID216583
UniProtKB IDP22939
UniProtKB Entry NameISPA_ECOLI
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0011073|Farnesyl diphosphate synthase (ispA)
ATGGACTTTCCGCAGCAACTCGAAGCCTGCGTTAAGCAGGCCAACCAGGCGCTGAGCCGT
TTTATCGCCCCACTGCCCTTTCAGAACACTCCCGTGGTCGAAACCATGCAGTATGGCGCA
TTATTAGGTGGTAAGCGCCTGCGACCTTTCCTGGTTTATGCCACCGGTCATATGTTCGGC
GTTAGCACAAACACGCTGGACGCACCCGCTGCCGCCGTTGAGTGTATCCACGCTTACTCA
TTAATTCATGATGATTTACCGGCAATGGATGATGACGATCTGCGTCGCGGTTTGCCAACC
TGCCATGTGAAGTTTGGCGAAGCAAACGCGATTCTCGCTGGCGACGCTTTACAAACGCTG
GCGTTCTCGATTTTAAGCGATGCCGATATGCCGGAAGTGTCGGACCGCGACAGAATTTCG
ATGATTTCTGAACTGGCGAGCGCCAGTGGTATTGCCGGAATGTGCGGTGGTCAGGCATTA
GATTTAGACGCGGAAGGCAAACACGTACCTCTGGACGCGCTTGAGCGTATTCATCGTCAT
AAAACCGGCGCATTGATTCGCGCCGCCGTTCGCCTTGGTGCATTAAGCGCCGGAGATAAA
GGACGTCGTGCTCTGCCGGTACTCGACAAGTATGCAGAGAGCATCGGCCTTGCCTTCCAG
GTTCAGGATGACATCCTGGATGTGGTGGGAGATACTGCAACGTTGGGAAAACGCCAGGGT
GCCGACCAGCAACTTGGTAAAAGTACCTACCCTGCACTTCTGGGTCTTGAGCAAGCCCGG
AAGAAAGCCCGGGATCTGATCGACGATGCCCGTCAGTCGCTGAAACAACTGGCTGAACAG
TCACTCGATACCTCGGCACTGGAAGCGCTAGCGGACTACATCATCCAGCGTAATAAATAA
GenBank Gene IDD00694
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Fujisaki S, Hara H, Nishimura Y, Horiuchi K, Nishino T: Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli. J Biochem. 1990 Dec;108(6):995-1000. 2089044
  2. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. 9278503
  3. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. 16738553
  4. Hosfield DJ, Zhang Y, Dougan DR, Broun A, Tari LW, Swanson RV, Finn J: Structural basis for bisphosphonate-mediated inhibition of isoprenoid biosynthesis. J Biol Chem. 2004 Mar 5;279(10):8526-9. Epub 2003 Dec 12. 14672944