T3DB: Sensor protein FixL

Name	Sensor protein FixL
Synonyms	2.7.13.3
Gene Name	fixL
Organism	Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Amino acid sequence	>lcl\|BSEQ0016493\|Sensor protein FixL MAPTRVTHPPDDGRGEHFRVRIEGFGVGTWDLDLKTWALDWSDTARTLLGIGQDQPASYD LFLSRLEPDDRERVESAIKRVSERGGGFDVSFRVAGTSNAGQWIRARAGLIRDEAGTARH LSGIFLDIDEEKQVEGALRTRETHLRSILHTIPDAMIVIDGHGIIQLFSTAAERLFGWSE LEAIGQNVNILMPEPDRSRHDSYISRYRTTSDPHIIGIGRIVTGKRRDGTTFPMHLSIGE MQSGGEPYFTGFVRDLTEHQQTQARLQELQSELVHVSRLSAMGEMASALAHELNQPLAAI SNYMKGSRRLLAGSSDPNTPKVESALDRAAEQALRAGQIIRRLRDFVARGESEKRVESLS KLIEEAGALGLAGAREQNVQLRFSLDPGADLVLADRVQIQQVLVNLFRNALEAMAQSQRR ELVVTNTPAADDMIEVEVSDTGSGFQDDVIPNLFQTFFTTKDTGMGVGLSISRSIIEAHG GRMWAESNASGGATFRFTLPAADEN
Number of residues	505
Molecular Weight	55651.92
Theoretical pI	5.38
GO Classification	Functions phosphorelay sensor kinase activity ATP binding metal ion binding Processes nitrogen fixation regulation of transcription, DNA-templated phosphorelay signal transduction system signal transduction by protein phosphorylation peptidyl-histidine phosphorylation Components intracellular
General Function	Phosphorelay sensor kinase activity
Specific Function	Putative oxygen sensor; modulates the activity of FixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates FixJ.
Pfam Domain Function	PAS (PF00989 ) HATPase_c (PF02518 ) HisKA (PF00512 )
Transmembrane Regions	Not Available
GenBank Protein ID	39523
UniProtKB ID	P23222
UniProtKB Entry Name	FIXL_BRADU
Cellular Location	Cell inner membrane; multi-pass membrane protein
Gene sequence	>lcl\|BSEQ0016494\|Sensor protein FixL (fixL) TTGGCGCCGACCCGCGTAACGCATCCGCCGGATGACGGCCGGGGCGAGCATTTCCGGGTC AGGATCGAGGGATTCGGCGTCGGCACCTGGGATCTCGACCTCAAGACCTGGGCGCTGGAC TGGTCGGATACCGCGCGGACCCTGCTCGGAATCGGGCAGGACCAGCCGGCGAGCTACGAC CTCTTCCTGTCACGCCTCGAGCCCGACGACCGCGAGCGCGTGGAGAGCGCGATCAAGCGC GTCTCCGAACGCGGTGGCGGCTTCGACGTGTCCTTCAGGGTCGCCGGCACCTCCAACGCA GGACAGTGGATTCGCGCCCGGGCCGGACTCATTCGGGACGAAGCCGGCACCGCCCGCCAT CTCAGCGGCATCTTTCTCGATATCGACGAGGAGAAGCAGGTCGAGGGTGCGCTCCGTACC CGCGAGACCCACCTCCGCTCGATCCTCCACACAATTCCCGACGCCATGATCGTCATCGAC GGCCACGGCATCATCCAGCTGTTCAGCACCGCCGCCGAGCGCCTGTTCGGCTGGTCCGAG CTCGAGGCGATCGGCCAGAACGTCAACATCCTGATGCCGGAGCCCGACCGCTCCCGGCAT GACAGCTACATTTCGCGTTACCGCACCACGAGCGATCCCCACATCATCGGCATCGGGCGC ATCGTGACCGGCAAGCGCCGCGACGGCACCACCTTCCCGATGCACCTGTCGATCGGCGAG ATGCAGTCCGGCGGCGAGCCCTATTTTACCGGTTTCGTCCGCGATCTCACCGAGCACCAG CAGACCCAGGCGCGTCTCCAGGAACTGCAATCCGAGCTCGTCCACGTCTCCAGGCTGAGC GCCATGGGCGAAATGGCGTCCGCGCTCGCGCACGAGCTCAACCAGCCGCTGGCGGCGATC AGCAACTACATGAAGGGCTCGCGGCGGCTGCTTGCCGGCAGCAGTGATCCGAACACACCG AAGGTCGAAAGCGCCCTGGACCGCGCCGCCGAGCAGGCGCTGCGCGCCGGCCAGATCATC CGGCGCCTGCGCGACTTCGTTGCCCGCGGCGAATCGGAGAAGCGGGTCGAGAGTCTCTCC AAGCTGATCGAGGAGGCCGGCGCGCTCGGGCTTGCCGGCGCGCGCGAGCAGAACGTGCAG CTCCGCTTCAGTCTCGATCCGGGCGCCGATCTCGTTCTCGCCGACCGGGTGCAGATCCAG CAGGTCCTGGTCAACCTGTTCCGCAACGCGCTGGAAGCGATGGCTCAGTCGCAGCGACGC GAGCTCGTCGTCACCAACACCCCCGCCGCCGACGACATGATCGAGGTCGAAGTGTCCGAC ACCGGCAGCGGTTTCCAGGACGACGTCATTCCGAACCTGTTTCAGACTTTCTTCACCACC AAGGACACCGGCATGGGCGTGGGACTGTCCATCAGCCGCTCGATCATCGAAGCTCACGGC GGGCGCATGTGGGCCGAGAGCAACGCATCGGGCGGGGCGACCTTCCGCTTCACCCTCCCG GCAGCCGACGAGAATTGA
GenBank Gene ID	X56808
GeneCard ID	Not Available
GenAtlas ID	Not Available
HGNC ID	Not Available
Chromosome Location	Not Available
Locus	Not Available
References	Anthamatten D, Hennecke H: The regulatory status of the fixL- and fixJ-like genes in Bradyrhizobium japonicum may be different from that in Rhizobium meliloti. Mol Gen Genet. 1991 Jan;225(1):38-48. 2000090 Kaneko T, Nakamura Y, Sato S, Minamisawa K, Uchiumi T, Sasamoto S, Watanabe A, Idesawa K, Iriguchi M, Kawashima K, Kohara M, Matsumoto M, Shimpo S, Tsuruoka H, Wada T, Yamada M, Tabata S: Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110. DNA Res. 2002 Dec 31;9(6):189-97. 12597275 Gong W, Hao B, Mansy SS, Gonzalez G, Gilles-Gonzalez MA, Chan MK: Structure of a biological oxygen sensor: a new mechanism for heme-driven signal transduction. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15177-82. 9860942 Gong W, Hao B, Chan MK: New mechanistic insights from structural studies of the oxygen-sensing domain of Bradyrhizobium japonicum FixL. Biochemistry. 2000 Apr 11;39(14):3955-62. 10747783