Chorismate pyruvate-lyase

NameChorismate pyruvate-lyase
Synonyms
  • 4.1.3.40
  • CL
Gene NameubiC
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0002992|Chorismate pyruvate-lyase
MSHPALTQLRALRYCKEIPALDPQLLDWLLLEDSMTKRFEQQGKTVSVTMIREGFVEQNE
IPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSGPELALQKLGKTPLGRYLF
TSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLPASPLY
Number of residues165
Molecular Weight18776.68
Theoretical pI8.2
GO Classification
Functions
  • chorismate lyase activity
Processes
  • pyruvate biosynthetic process
  • ubiquinone biosynthetic process
Components
  • cytosol
General FunctionChorismate lyase activity
Specific FunctionRemoves the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID43231
UniProtKB IDP26602
UniProtKB Entry NameUBIC_ECOLI
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0021294|Chorismate pyruvate-lyase (ubiC)
ATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCC
CTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAA
CAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAA
ATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTACTGGTTACGTGAAATTTTG
TTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTA
AGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTC
ACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGG
CGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTAACAGAACTGTTTTTACCG
GCGTCACCGTTGTACTAA
GenBank Gene IDX66619
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Siebert M, Bechthold A, Melzer M, May U, Berger U, Schroder G, Schroder J, Severin K, Heide L: Ubiquinone biosynthesis. Cloning of the genes coding for chorismate pyruvate-lyase and 4-hydroxybenzoate octaprenyl transferase from Escherichia coli. FEBS Lett. 1992 Aug 3;307(3):347-50. 1644192
  2. Nichols BP, Green JM: Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase. J Bacteriol. 1992 Aug;174(16):5309-16. 1644758
  3. Nishimura K, Nakahigashi K, Inokuchi H: Location of the ubiA gene on the physical map of Escherichia coli. J Bacteriol. 1992 Sep;174(17):5762. 1512213
  4. Wu G, Williams HD, Gibson F, Poole RK: Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase. J Gen Microbiol. 1993 Aug;139(8):1795-805. 8409922
  5. Blattner FR, Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL: Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 1993 Nov 25;21(23):5408-17. 8265357
  6. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. 9278503
  7. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. 16738553
  8. Siebert M, Severin K, Heide L: Formation of 4-hydroxybenzoate in Escherichia coli: characterization of the ubiC gene and its encoded enzyme chorismate pyruvate-lyase. Microbiology. 1994 Apr;140 ( Pt 4):897-904. 8012607
  9. Holden MJ, Mayhew MP, Gallagher DT, Vilker VL: Chorismate lyase: kinetics and engineering for stability. Biochim Biophys Acta. 2002 Jan 31;1594(1):160-7. 11825618
  10. Stover C, Mayhew MP, Holden MJ, Howard A, Gallagher DT: Crystallization and 1.1-A diffraction of chorismate lyase from Escherichia coli. J Struct Biol. 2000 Feb;129(1):96-9. 10675300
  11. Gallagher DT, Mayhew M, Holden MJ, Howard A, Kim KJ, Vilker VL: The crystal structure of chorismate lyase shows a new fold and a tightly retained product. Proteins. 2001 Aug 15;44(3):304-11. 11455603
  12. Smith N, Roitberg AE, Rivera E, Howard A, Holden MJ, Mayhew M, Kaistha S, Gallagher DT: Structural analysis of ligand binding and catalysis in chorismate lyase. Arch Biochem Biophys. 2006 Jan 1;445(1):72-80. Epub 2005 Nov 22. 16343413