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NameDNA-directed RNA polymerases I, II, and III subunit RPABC2
Synonyms
  • DNA-directed RNA polymerase II subunit F
  • DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide
  • POLRF
  • RNA polymerases I, II, and III subunit ABC2
  • RPABC14.4
  • RPB14.4
  • RPB6 homolog
  • RPC15
Gene NamePOLR2F
OrganismHuman
Amino acid sequence
>lcl|BSEQ0013575|DNA-directed RNA polymerases I, II, and III subunit RPABC2
MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKY
ERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGV
DELIITD
Number of residues127
Molecular Weight14477.92
Theoretical pINot Available
GO Classification
Functions
  • DNA-directed RNA polymerase activity
  • DNA binding
Processes
  • transcription from RNA polymerase III promoter
  • regulation of gene expression, epigenetic
  • DNA repair
  • transcription initiation from RNA polymerase I promoter
  • positive regulation of type I interferon production
  • 7-methylguanosine mRNA capping
  • nucleotide-excision repair
  • gene silencing by RNA
  • transcription from RNA polymerase II promoter
  • mRNA splicing, via spliceosome
  • piRNA metabolic process
  • gene expression
  • positive regulation of viral transcription
  • transcription initiation from RNA polymerase II promoter
  • RNA splicing
  • transcription elongation from RNA polymerase II promoter
  • termination of RNA polymerase I transcription
  • innate immune response
  • termination of RNA polymerase III transcription
  • somatic stem cell population maintenance
  • transcription elongation from RNA polymerase I promoter
  • transcription-coupled nucleotide-excision repair
  • transcription elongation from RNA polymerase III promoter
  • viral process
  • transcription from RNA polymerase I promoter
  • negative regulation of gene expression, epigenetic
Components
  • nucleus
  • DNA-directed RNA polymerase II, core complex
  • cytosol
  • DNA-directed RNA polymerase I complex
  • nucleolus
  • DNA-directed RNA polymerase III complex
  • nucleoplasm
General FunctionDna-directed rna polymerase activity
Specific FunctionDNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity).
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDP61218
UniProtKB Entry NameRPAB2_HUMAN
Cellular LocationNucleus
Gene sequenceNot Available
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:9193
Chromosome LocationNot Available
LocusNot Available
References
  1. Acker J, Wintzerith M, Vigneron M, Kedinger C: A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper. DNA Seq. 1994;4(5):329-31. 7803819
  2. Pusch C, Wang Z, Roe B, Blin N: Genomic structure of the RNA polymerase II small subunit (hRPB14.4) locus (POLRF) and mapping to 22q13.1 by sequence identity. Genomics. 1996 Jun 15;34(3):440-2. 8786150
  3. Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I: A genome annotation-driven approach to cloning the human ORFeome. Genome Biol. 2004;5(10):R84. Epub 2004 Sep 30. 15461802
  4. Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP, et al.: The DNA sequence of human chromosome 22. Nature. 1999 Dec 2;402(6761):489-95. 10591208
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  6. Kershnar E, Wu SY, Chiang CM: Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes. J Biol Chem. 1998 Dec 18;273(51):34444-53. 9852112
  7. Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3. doi: 10.1126/scisignal.2000475. 20068231
  8. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. 24275569
  9. del Rio-Portilla F, Gaskell A, Gilbert D, Ladias JA, Wagner G: Solution structure of the hRPABC14.4 subunit of human RNA polymerases. Nat Struct Biol. 1999 Nov;6(11):1039-42. 10542096
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