T3DB: Malate dehydrogenase

Name	Malate dehydrogenase
Synonyms	1.1.1.37
Gene Name	mdh
Organism	Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
Amino acid sequence	>lcl\|BSEQ0016371\|Malate dehydrogenase MRKKISIIGAGFVGSTTAHWLAAKELGDIVLLDFVEGVPQGKALDLYEASPIEGFDVRVT GTNNYADTANSDVIVVTSGAPRKPGMSREDLIKVNADITRACISQAAPLSPNAVIIMVNN PLDAMTYLAAEVSGFPKERVIGQAGVLDAARYRTFIAMEAGVSVEDVQAMLMGGHGDEMV PLPRFSTISGIPVSEFIAPDRLAQIVERTRKGGGEIVNLLKTGSAYYAPAAATAQMVEAV LKDKKRVMPVAAYLTGQYGLNDIYFGVPVILGAGGVEKILELPLNEEEMALLNASAKAVR ATLDTLKSL
Number of residues	309
Molecular Weight	32750.54
Theoretical pI	4.91
GO Classification	Functions L-malate dehydrogenase activity Processes malate metabolic process tricarboxylic acid cycle carbohydrate metabolic process
General Function	L-malate dehydrogenase activity
Specific Function	Catalyzes the reversible oxidation of malate to oxaloacetate.
Pfam Domain Function	Ldh_1_C (PF02866 ) Ldh_1_N (PF00056 )
Transmembrane Regions	Not Available
GenBank Protein ID	1491644
UniProtKB ID	P80040
UniProtKB Entry Name	MDH_CHLAA
Cellular Location	Not Available
Gene sequence	>lcl\|BSEQ0016372\|Malate dehydrogenase (mdh) ATGCGCAAGAAGATTAGTATTATCGGGGCAGGATTTGTCGGTTCGACGACAGCTCACTGG CTGGCCGCTAAAGAATTAGGTGACATTGTTCTGCTTGATTTTGTCGAGGGTGTGCCTCAA GGCAAGGCTCTCGATCTCTACGAGGCATCACCAATTGAAGGGTTTGATGTTCGGGTGACC GGCACGAACAACTACGCCGATACTGCCAACTCGGATGTCATCGTTGTTACGTCAGGTGCT CCGCGCAAGCCGGGCATGAGCCGTGAGGATTTGATCAAGGTCAACGCCGACATCACCCGT GCCTGTATCAGTCAGGCTGCCCCGCTCTCGCCGAATGCCGTGATTATCATGGTCAATAAT CCTCTGGATGCGATGACCTATCTGGCGGCGGAGGTTAGTGGCTTCCCCAAAGAGCGTGTC ATTGGTCAGGCCGGCGTCCTCGATGCGGCCCGCTATCGCACCTTCATTGCCATGGAGGCG GGTGTTTCGGTTGAAGATGTTCAGGCGATGCTGATGGGTGGTCACGGCGATGAGATGGTA CCGCTGCCCCGGTTTTCAACCATTAGCGGTATTCCGGTGAGCGAGTTCATTGCGCCTGAT CGTCTGGCTCAGATTGTTGAGCGCACCCGGAAGGGTGGTGGCGAGATTGTGAATCTGCTG AAGACCGGCAGTGCGTATTATGCTCCCGCCGCCGCCACAGCGCAGATGGTCGAAGCGGTG CTCAAAGACAAGAAGCGGGTCATGCCGGTGGCAGCCTACCTGACCGGTCAGTACGGCCTG AATGATATTTACTTTGGTGTACCGGTGATCCTCGGTGCCGGTGGAGTAGAGAAAATTCTC GAATTGCCGCTCAACGAAGAGGAGATGGCACTGCTGAACGCATCGGCGAAAGCGGTACGT GCCACCCTCGACACGCTGAAGTCTCTCTAA
GenBank Gene ID	X89038
GeneCard ID	Not Available
GenAtlas ID	Not Available
HGNC ID	Not Available
Chromosome Location	Not Available
Locus	Not Available
References	Synstad B, Emmerhoff O, Sirevag R: Malate dehydrogenase from the green gliding bacterium Chloroflexus aurantiacus is phylogenetically related to lactic dehydrogenases. Arch Microbiol. 1996 May;165(5):346-53. 8661927 Tang KH, Barry K, Chertkov O, Dalin E, Han CS, Hauser LJ, Honchak BM, Karbach LE, Land ML, Lapidus A, Larimer FW, Mikhailova N, Pitluck S, Pierson BK, Blankenship RE: Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus. BMC Genomics. 2011 Jun 29;12:334. doi: 10.1186/1471-2164-12-334. 21714912 Rolstad AK, Howland E, Sirevag R: Malate dehydrogenase from the thermophilic green bacterium Chloroflexus aurantiacus: purification, molecular weight, amino acid composition, and partial amino acid sequence. J Bacteriol. 1988 Jul;170(7):2947-53. 3133356 Dalhus B, Saarinen M, Sauer UH, Eklund P, Johansson K, Karlsson A, Ramaswamy S, Bjork A, Synstad B, Naterstad K, Sirevag R, Eklund H: Structural basis for thermophilic protein stability: structures of thermophilic and mesophilic malate dehydrogenases. J Mol Biol. 2002 May 3;318(3):707-21. 12054817 Bjork A, Dalhus B, Mantzilas D, Eijsink VG, Sirevag R: Stabilization of a tetrameric malate dehydrogenase by introduction of a disulfide bridge at the dimer-dimer interface. J Mol Biol. 2003 Dec 5;334(4):811-21. 14636605