2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase

Name2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
SynonymsNot Available
Gene NamecumD
OrganismPseudomonas fluorescens
Amino acid sequence
>lcl|BSEQ0022031|2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
MANLEIGKSILAAGVLTNYHDVGEGQPVILIHGSGPGVSAYANWRLTIPALSKFYRVIAP
DMVGFGFTDRPENYNYSKDSWVDHIIGIMDALEIEKAHIVGNSFGGGLAIATALRYSERV
DRMVLMGAAGTRFDVTEGLNAVWGYTPSIENMRNLLDIFAYDRSLVTDELARLRYEASIQ
PGFQESFSSMFPEPRQRWIDALASSDEDIKTLPNETLIIHGREDQVVPLSSSLRLGELID
RAQLHVFGRCGHWTQIEQTDRFNRLVVEFFNEANTPKLVGRP
Number of residues282
Molecular Weight31489.385
Theoretical pI5.03
GO Classification
Functions
  • hydrolase activity
General FunctionHydrolase activity
Specific FunctionNot Available
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDP96965
UniProtKB Entry NameP96965_PSEFL
Cellular LocationCytoplasmic
Gene sequence
>lcl|BSEQ0005375|849 bp
ATGGCAAACCTGGAAATAGGTAAAAGTATTTTAGCTGCAGGGGTTCTAACAAACTATCAC
GATGTCGGGGAGGGACAGCCTGTGATACTAATTCATGGTTCTGGGCCCGGCGTGAGCGCT
TATGCGAATTGGCGACTGACCATTCCTGCTCTGAGCAAGTTTTATCGTGTTATCGCCCCT
GATATGGTTGGTTTTGGCTTTACGGATCGACCTGAAAACTATAATTATTCCAAGGATAGT
TGGGTTGACCATATAATCGGTATTATGGACGCCCTGGAAATAGAAAAGGCTCACATCGTA
GGTAACTCATTCGGTGGGGGGCTGGCGATTGCTACCGCGCTTCGTTATTCGGAGCGCGTT
GATCGTATGGTTCTGATGGGGGCGGCCGGTACTCGCTTTGACGTGACAGAGGGCCTGAAC
GCGGTGTGGGGTTACACGCCGTCGATAGAAAACATGCGTAATCTCCTCGATATATTCGCT
TATGATCGCTCCTTAGTCACCGACGAATTAGCGAGACTTCGATATGAGGCGAGTATTCAG
CCAGGATTTCAAGAGTCATTCTCCAGCATGTTTCCTGAGCCGCGCCAACGCTGGATAGAC
GCGTTGGCTAGCTCGGATGAAGATATCAAGACTCTTCCCAATGAGACACTGATTATTCAT
GGTCGAGAGGATCAGGTTGTTCCTCTATCCAGCAGTCTGCGTCTAGGCGAACTTATCGAC
CGGGCGCAGTTGCATGTTTTCGGGCGATGTGGGCACTGGACACAAATTGAGCAGACTGAC
CGATTCAATCGTCTTGTGGTCGAGTTTTTTAATGAGGCCAACACACCTAAACTAGTGGGT
CGTCCGTAA
GenBank Gene IDD83955
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Fushinobu S, Saku T, Hidaka M, Jun SY, Nojiri H, Yamane H, Shoun H, Omori T, Wakagi T: Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products. Protein Sci. 2002 Sep;11(9):2184-95. 12192074
  2. Fushinobu S, Jun SY, Hidaka M, Nojiri H, Yamane H, Shoun H, Omori T, Wakagi T: A series of crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with various cleavage products. Biosci Biotechnol Biochem. 2005 Mar;69(3):491-8. 15784976
  3. Jun SY, Fushinobu S, Nojiri H, Omori T, Shoun H, Wakagi T: Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01. Biochim Biophys Acta. 2006 Jul;1764(7):1159-66. Epub 2006 Jun 7. 16844437