Octanoyltransferase

NameOctanoyltransferase
Synonyms
  • 2.3.1.181
  • Lipoate-protein ligase B
  • Lipoyl/octanoyl transferase
  • Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase
Gene NamelipB
OrganismMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Amino acid sequence
>lcl|BSEQ0051229|Octanoyltransferase
MTGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTE
THERPIDGTPVVDTDRGGKITWHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADL
GLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFALNCDCDLAAFTAIVPCGISD
AAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL
Number of residues230
Molecular Weight24210.415
Theoretical pINot Available
GO Classification
Functions
  • lipoyl(octanoyl) transferase activity
  • octanoyltransferase activity
Processes
  • growth
  • protein lipoylation
  • lipoate biosynthetic process
Components
  • cytoplasm
  • plasma membrane
General FunctionCatalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.
Specific FunctionLipoyl(octanoyl) transferase activity
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDP9WK83
UniProtKB Entry NameLIPB_MYCTU
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0051230|Octanoyltransferase (lipB)
GTGACGGGTTCTATCCGGTCGAAGCTGTCCGCGATCGACGTCCGCCAGCTGGGGACCGTC
GACTACCGGACCGCGTGGCAGCTACAGCGAGAGCTAGCCGACGCCCGGGTCGCCGGCGGC
GCCGACACGCTGCTGCTGTTGGAACACCCCGCGGTCTACACCGCCGGACGGCGTACCGAG
ACACACGAGCGACCCATTGACGGCACTCCGGTCGTCGACACCGACCGCGGCGGCAAGATC
ACCTGGCACGGTCCGGGGCAATTGGTCGGCTACCCGATCATCGGGCTGGCCGAACCCCTC
GACGTGGTCAATTACGTTCGGCGCCTTGAAGAATCGCTGATCCAAGTCTGCGCCGATCTG
GGCCTGCACGCCGGCCGCGTCGACGGCCGGTCCGGGGTCTGGCTGCCCGGCAGGCCGGCG
CGCAAGGTCGCGGCCATCGGTGTCCGGGTGTCGCGGGCGACGACACTGCACGGGTTTGCG
CTCAACTGCGATTGTGATTTGGCTGCCTTCACCGCCATCGTGCCATGCGGAATCAGTGAC
GCCGCAGTGACATCGCTGTCCGCCGAACTCGGCCGTACGGTCACCGTCGACGAGGTCCGC
GCGACGGTCGCCGCCGCTGTCTGCGCCGCTCTGGACGGCGTCCTACCGGTCGGTGACCGC
GTGCCCTCACACGCCGTACCATCGCCGTTATGA
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Krogh A, McLean J, Moule S, Murphy L, Oliver K, Osborne J, Quail MA, Rajandream MA, Rogers J, Rutter S, Seeger K, Skelton J, Squares R, Squares S, Sulston JE, Taylor K, Whitehead S, Barrell BG: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 1998 Jun 11;393(6685):537-44. 9634230
  2. Kelkar DS, Kumar D, Kumar P, Balakrishnan L, Muthusamy B, Yadav AK, Shrivastava P, Marimuthu A, Anand S, Sundaram H, Kingsbury R, Harsha HC, Nair B, Prasad TS, Chauhan DS, Katoch K, Katoch VM, Kumar P, Chaerkady R, Ramachandran S, Dash D, Pandey A: Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3. 21969609
  3. Ma Q, Zhao X, Nasser Eddine A, Geerlof A, Li X, Cronan JE, Kaufmann SH, Wilmanns M: The Mycobacterium tuberculosis LipB enzyme functions as a cysteine/lysine dyad acyltransferase. Proc Natl Acad Sci U S A. 2006 Jun 6;103(23):8662-7. Epub 2006 May 30. 16735476