T3DB: Clavaminate synthase 1

Name	Clavaminate synthase 1
Synonyms	1.14.11.21 CAS1 Clavaminic acid synthase 1 CS1
Gene Name	cs1
Organism	Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Amino acid sequence	>lcl\|BSEQ0010917\|Clavaminate synthase 1 MTSVDCTAYGPELRALAARLPRTPRADLYAFLDAAHTAAASLPGALATALDTFNAEGSED GHLLLRGLPVEADADLPTTPSSTPAPEDRSLLTMEAMLGLVGRRLGLHTGYRELRSGTVY HDVYPSPGAHHLSSETSETLLEFHTEMAYHRLQPNYVMLACSRADHERTAATLVASVRKA LPLLDERTRARLLDRRMPCCVDVAFRGGVDDPGAIAQVKPLYGDADDPFLGYDRELLAPE DPADKEAVAALSKALDEVTEAVYLEPGDLLIVDNFRTTHARTPFSPRWDGKDRWLHRVYI RTDRNGQLSGGERAGDVVAFTPRG
Number of residues	324
Molecular Weight	35369.325
Theoretical pI	5.24
GO Classification	Functions iron ion binding clavaminate synthase activity Processes antibiotic biosynthetic process clavulanic acid biosynthetic process
General Function	Iron ion binding
Specific Function	Not Available
Pfam Domain Function	TauD (PF02668 )
Transmembrane Regions	Not Available
GenBank Protein ID	153220
UniProtKB ID	Q05581
UniProtKB Entry Name	CAS1_STRC2
Cellular Location	Not Available
Gene sequence	>lcl\|BSEQ0002609\|975 bp ATGACCTCAGTGGACTGCACCGCGTACGGCCCCGAGCTGCGCGCGCTCGCCGCCCGGCTG CCCCGGACCCCCCGGGCCGACCTGTACGCCTTCCTGGACGCCGCGCACACAGCCGCCGCC TCGCTCCCCGGCGCCCTCGCCACCGCGCTGGACACCTTCAACGCCGAGGGCAGCGAGGAC GGCCATCTGCTGCTGCGCGGCCTCCCGGTGGAGGCCGACGCCGACCTCCCCACCACCCCG AGCAGCACCCCGGCGCCCGAGGACCGCTCCCTGCTGACCATGGAGGCCATGCTCGGACTG GTGGGCCGCCGGCTCGGTCTGCACACGGGGTACCGGGAGCTGCGCTCGGGCACGGTCTAC CACGACGTGTACCCGTCGCCCGGCGCGCACCACCTGTCCTCGGAGACCTCCGAGACGCTG CTGGAGTTCCACACGGAGATGGCCTACCACCGGCTCCAGCCGAACTACGTCATGCTGGCC TGCTCCCGGGCCGACCACGAGCGCACGGCGGCCACACTCGTCGCCTCGGTCCGCAAGGCG CTGCCCCTGCTGGACGAGAGGACCCGGGCCCGGCTCCTCGACCGGAGGATGCCCTGCTGC GTGGATGTGGCCTTCCGCGGCGGGGTGGACGACCCGGGCGCCATCGCCCAGGTCAAACCG CTCTACGGGGACGCGGACGATCCCTTCCTCGGGTACGACCGCGAGCTGCTGGCGCCGGAG GACCCCGCGGACAAGGAGGCCGTCGCCGCCCTGTCCAAGGCGCTCGACGAGGTCACGGAG GCGGTGTATCTGGAGCCCGGCGATCTGCTGATCGTCGACAACTTCCGCACCACGCACGCG CGGACGCCGTTCTCGCCCCGCTGGGACGGGAAGGACCGCTGGCTGCACCGCGTCTACATC CGCACCGACCGCAATGGACAGCTCTCCGGCGGCGAGCGCGCGGGCGACGTCGTCGCCTTC ACACCGCGCGGCTGA
GenBank Gene ID	L06213
GeneCard ID	Not Available
GenAtlas ID	Not Available
HGNC ID	Not Available
Chromosome Location	Not Available
Locus	Not Available
References	Marsh EN, Chang MD, Townsend CA: Two isozymes of clavaminate synthase central to clavulanic acid formation: cloning and sequencing of both genes from Streptomyces clavuligerus. Biochemistry. 1992 Dec 22;31(50):12648-57. 1472501 Busby RW, Chang MD, Busby RC, Wimp J, Townsend CA: Expression and purification of two isozymes of clavaminate synthase and initial characterization of the iron binding site. General error analysis in polymerase chain reaction amplification. J Biol Chem. 1995 Mar 3;270(9):4262-9. 7876185 Zhang Z, Ren J, Stammers DK, Baldwin JE, Harlos K, Schofield CJ: Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase. Nat Struct Biol. 2000 Feb;7(2):127-33. 10655615 Zhang Z, Ren Js, Harlos K, McKinnon CH, Clifton IJ, Schofield CJ: Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements. FEBS Lett. 2002 Apr 24;517(1-3):7-12. 12062399