Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase

NameNicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Synonyms
  • 2.4.2.21
  • N(1)-alpha-phosphoribosyltransferase
  • NN:DBI PRT
Gene NamecobT
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Amino acid sequence
>lcl|BSEQ0002447|Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
MQTLHALLRDIPAPDAEAMARAQQHIDGLLKPPGSLGRLETLAVQLAGMPGLNGTPQVGE
KAVLVMCADHGVWDEGVAVSPKIVTAIQAANMTRGTTGVCVLAAQAGAKVHVIDVGIDAE
PIPGVVNMRVARGCGNIAVGPAMSRLQAEALLLEVSRYTCDLAQRGVTLFGVGELGMANT
TPAAAMVSVFTGSDAKEVVGIGANLPPSRIDNKVDVVRRAIAINQPNPRDGIDVLSKVGG
FDLVGMTGVMLGAARCGLPVLLDGFLSYSAALAACQIAPAVRPYLIPSHFSAEKGARIAL
AHLSMEPYLHMAMRLGEGSGAALAMPIVEAACAMFHNMGELAASNIVLPEGNANAT
Number of residues356
Molecular Weight36612.305
Theoretical pI6.3
GO Classification
Functions
  • nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity
  • cobalamin-transporting ATPase activity
Processes
  • nucleoside biosynthetic process
  • cobalamin biosynthetic process
General FunctionNicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity
Specific FunctionCatalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID535767
UniProtKB IDQ05603
UniProtKB Entry NameCOBT_SALTY
Cellular LocationNot Available
Gene sequence
>lcl|BSEQ0016358|Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT)
ATGCAGACACTACACGCTTTACTCCGTGACATTCCTGCGCCGGACGCCGAGGCGATGGCG
CGTGCGCAGCAACATATTGACGGCCTGCTCAAACCGCCGGGCAGCCTGGGCAGACTGGAA
ACCTTAGCCGTTCAGCTCGCGGGTATGCCGGGTCTTAACGGTACGCCGCAGGTAGGTGAA
AAGGCGGTGCTGGTGATGTGCGCCGACCATGGCGTCTGGGATGAAGGCGTAGCGGTTTCG
CCCAAAATCGTGACGGCGATTCAGGCGGCGAATATGACGCGGGGAACAACCGGCGTATGC
GTGCTTGCCGCTCAGGCCGGTGCGAAGGTGCATGTCATTGATGTCGGTATTGATGCCGAA
CCTATTCCTGGCGTAGTTAATATGCGCGTCGCGCGCGGCTGCGGAAATATTGCCGTTGGC
CCGGCGATGAGTCGCTTGCAGGCTGAGGCGCTTTTGCTGGAGGTTTCCCGCTACACCTGC
GACCTGGCGCAACGCGGCGTGACCTTATTTGGCGTAGGGGAGCTGGGAATGGCGAACACT
ACGCCAGCCGCCGCGATGGTCAGCGTTTTTACAGGAAGTGATGCAAAAGAGGTGGTGGGG
ATTGGCGCGAATCTTCCGCCTTCCCGCATCGATAATAAAGTGGACGTCGTGCGGCGGGCG
ATTGCGATTAATCAGCCCAATCCGCGCGACGGCATTGATGTGTTGTCGAAGGTGGGTGGT
TTTGATCTGGTCGGGATGACCGGCGTGATGCTTGGCGCGGCAAGGTGTGGCTTACCCGTA
TTGCTGGATGGCTTTCTTTCCTACTCGGCAGCGCTGGCGGCCTGTCAGATTGCGCCTGCG
GTGAGACCTTATCTGATCCCGTCGCACTTTTCGGCGGAAAAGGGCGCCCGCATCGCGCTC
GCGCATTTGTCTATGGAACCCTATTTGCATATGGCGATGCGGTTAGGTGAAGGAAGCGGC
GCGGCGCTGGCGATGCCGATCGTGGAAGCCGCGTGCGCCATGTTCCACAACATGGGGGAG
TTGGCGGCCAGTAATATTGTCCTGCCGGAGGGGAACGCAAACGCAACATAA
GenBank Gene IDL35477
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Chen P, Ailion M, Weyand N, Roth J: The end of the cob operon: evidence that the last gene (cobT) catalyzes synthesis of the lower ligand of vitamin B12, dimethylbenzimidazole. J Bacteriol. 1995 Mar;177(6):1461-9. 7883701
  2. McClelland M, Sanderson KE, Spieth J, Clifton SW, Latreille P, Courtney L, Porwollik S, Ali J, Dante M, Du F, Hou S, Layman D, Leonard S, Nguyen C, Scott K, Holmes A, Grewal N, Mulvaney E, Ryan E, Sun H, Florea L, Miller W, Stoneking T, Nhan M, Waterston R, Wilson RK: Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature. 2001 Oct 25;413(6858):852-6. 11677609
  3. Roth JR, Lawrence JG, Rubenfield M, Kieffer-Higgins S, Church GM: Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium. J Bacteriol. 1993 Jun;175(11):3303-16. 8501034
  4. Cheong CG, Escalante-Semerena JC, Rayment I: The three-dimensional structures of nicotinate mononucleotide:5,6- dimethylbenzimidazole phosphoribosyltransferase (CobT) from Salmonella typhimurium complexed with 5,6-dimethybenzimidazole and its reaction products determined to 1.9 A resolution. Biochemistry. 1999 Dec 7;38(49):16125-35. 10587435