Carboxylesterase

NameCarboxylesterase
Synonyms
  • 3.1.1.1
  • est30
Gene Nameest
OrganismGeobacillus stearothermophilus
Amino acid sequence
>lcl|BSEQ0037140|Carboxylesterase
MKIVPPKPFFFEAGERAVLLLHGFTGNSADVRMLGRFLESKGYTCHAPIYKGHGVPPEEL
VHTGPDDWWQDVMNGYEFLKNKGYEKIAVAGLSLGGVFSLKLGYTVPIEGIVTMCAPMYI
KSEETMYEGVLEYAREYKKREGKSEEQIEQEMEKFKQTPMKTLKALQELIADVRDHLDLI
YAPTFVVQARHDEMINPDSANIIYNEIESPVKQIKWYEQSGHVITLDQEKDQLHEDIYAF
LESLDW
Number of residues246
Molecular Weight28256.125
Theoretical pI4.79
GO Classification
Functions
  • carboxylic ester hydrolase activity
General FunctionCarboxylic ester hydrolase activity
Specific FunctionInvolved in the detoxification of xenobiotics. Shows maximal activity with C6 substrates, with gradually decreasing activity from C8 to C12 substrates. No activity for higher chain length substrates acids rather than long-chain ones.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID216314
UniProtKB IDQ06174
UniProtKB Entry NameEST_GEOSE
Cellular LocationNot Available
Gene sequence
>lcl|BSEQ0002518|744 bp
ATGATGAAAATTGTTCCGCCGAAGCCGTTTTTCTTTGAAGCCGGGGAGCGGGCGGTGCTG
CTGTTGCATGGGTTTACCGGCAATTCCGCTGATGTTCGGATGCTCGGGCGTTTTTTAGAA
TCCAAAGGCTATACGTGCCATGCGCCTATTTACAAAGGACACGGCGTGCCGCCTGAGGAG
CTCGTCCACACCGGGCCGGATGACTGGTGGCAAGATGTCATGAACGGCTACGAGTTTTTG
AAAAACAAGGGCTACGAAAAAATCGCCGTCGCCGGACTGTCGCTTGGAGGCGTATTTTCA
TTGAAATTAGGTTACACTGTACCTATAGAGGGCATTGTGACGATGTGCGCGCCGATGTAC
ATCAAAAGCGAGGAAACGATGTACGAAGGCGTGCTCGAGTATGCGCGCGAGTATAAAAAG
CGGGAAGGAAAATCAGAGGAGCAGATCGAACAGGAGATGGAGAAGTTCAAGCAGACGCCG
ATGAAGACGTTAAAGGCGCTGCAGGAGCTGATCGCCGATGTGCGTGACCATCTTGATTTG
ATTTATGCCCCGACGTTTGTTGTGCAGGCGCGCCATGATGAGATGATCAACCCGGACAGC
GCGAACATCATTTATAACGAAATTGAATCGCCGGTCAAACAAATCAAGTGGTATGAGCAA
TCAGGCCATGTGATTACGCTTGATCAAGAAAAAGATCAGCTGCATGAAGATATTTATGCA
TTTCTTGAATCGTTAGATTGGTAA
GenBank Gene IDD12681
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. Kugimiya W, Otani Y, Hashimoto Y: Molecular cloning and structure of the gene for esterase from a thermophilic bacterium, Bacillus stearothermophilus IFO 12550. Biosci Biotechnol Biochem. 1992 Dec;56(12):2074-5. 1369099
  2. Ewis HE, Abdelal AT, Lu CD: Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus. Gene. 2004 Mar 31;329:187-95. 15033540
  3. Liu P, Wang YF, Ewis HE, Abdelal AT, Lu CD, Harrison RW, Weber IT: Covalent reaction intermediate revealed in crystal structure of the Geobacillus stearothermophilus carboxylesterase Est30. J Mol Biol. 2004 Sep 10;342(2):551-61. 15327954