(S)-2-haloacid dehalogenase

Name(S)-2-haloacid dehalogenase
Synonyms
  • 2-haloalkanoic acid dehalogenase
  • 3.8.1.2
  • Halocarboxylic acid halidohydrolase
  • L-2-haloacid dehalogenase
Gene NamedhlB
OrganismXanthobacter autotrophicus
Amino acid sequence
>lcl|BSEQ0011144|(S)-2-haloacid dehalogenase
MIKAVVFDAYGTLFDVQSVADATERAYPGRGEYITQVWRQKQLEYSWLRALMGRYADFWG
VTREALAYTLGTLGLEPDESFLADMAQAYNRLTPYPDAAQCLAELAPLKRAILSNGAPDM
LQALVANAGLTDSFDAVISVDAKRVFKPHPDSYALVEEVLGVTPAEVLFVSSNGFDVGGA
KNFGFSVARVARLSQEALARELVSGTIAPLTMFKALRMREETYAEAPDFVVPALGDLPRL
VRGMAGAHLAPAV
Number of residues253
Molecular Weight27469.15
Theoretical pI4.71
GO Classification
Functions
  • (S)-2-haloacid dehalogenase activity
General Function(s)-2-haloacid dehalogenase activity
Specific FunctionCatalyzes the hydrolytic dehalogenation of small L-2-haloalkanoic acids to yield the corresponding D-2-hydroxyalkanoic acids. Active with 2-halogenated carboxylic acids and converts only the L-isomer of 2-chloropropionic acid with inversion of configuration to produce D-lactate.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID155350
UniProtKB IDQ60099
UniProtKB Entry NameHAD_XANAU
Cellular LocationCytoplasmic
Gene sequence
>lcl|BSEQ0003200|762 bp
ATGATCAAGGCAGTCGTGTTCGACGCTTACGGTACGCTCTTCGACGTCCAGTCGGTGGCC
GACGCCACCGAGCGGGCGTATCCAGGCCGGGGCGAGTACATCACGCAGGTCTGGCGGCAG
AAGCAGCTGGAATACAGCTGGCTCCGCGCGCTGATGGGGCGCTATGCCGACTTTTGGGGC
GTCACGCGGGAAGCGCTGGCCTATACCCTCGGAACGCTGGGGCTGGAGCCGGACGAGTCC
TTCCTCGCCGGGATGGCGCAGGCCTACAACCGCCTCACGCCCTATCCGGACGCCGCGCAA
TGCCTCGCGGAGCTGGCGCCCCTCAAGCGCGCCATCCTCTCCAACGGCGCGCCCGACATG
CTGCAGGCGCTCGTGGCCAATGCGGGCCTGACGGACAGCTTCGATGCCGTCATCAGCGTC
GATGCCAAGCGCGTGTTCAAGCCTCATCCCGACTCCTACGCGCTGGTGGAGGAGGTACTA
GGCGTGACGCCCGCGGAGGTGCTGTTCGTGTCCTCCAACGGCTTCGACGTCGGCGGCGCG
AAGAATTTCGGCTTCAGCGTCGCCCGGGTCGCGCGCCTGTCGCAGGAGGCGCTGGCGCGC
GAACTCGTCTCGGGTACCATCGCGCCCCTGACCATGTTCAAGGCGCTGAGGATGCGGGAA
GAAACCTATGCGGAGGCGCCTGATTTCGTGGTGCCCGCCCTTGGCGACCTGCCGCGGCTG
GTTCGCGGGATGGCCGGCGCTCATCTCGCACCAGCGGTGTGA
GenBank Gene IDM81691
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDNot Available
Chromosome LocationNot Available
LocusNot Available
References
  1. van der Ploeg J, van Hall G, Janssen DB: Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene. J Bacteriol. 1991 Dec;173(24):7925-33. 1744048
  2. van der Ploeg J, Janssen DB: Sequence analysis of the upstream region of dhlB, the gene encoding haloalkanoic acid dehalogenase of Xanthobacter autotrophicus GJ10. Biodegradation. 1995 Sep;6(3):257-63. 7580000
  3. Ridder IS, Rozeboom HJ, Kalk KH, Janssen DB, Dijkstra BW: Three-dimensional structure of L-2-haloacid dehalogenase from Xanthobacter autotrophicus GJ10 complexed with the substrate-analogue formate. J Biol Chem. 1997 Dec 26;272(52):33015-22. 9407083